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EC number: 203-234-3 | CAS number: 104-76-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-ethylhexan-1-ol
- EC Number:
- 203-234-3
- EC Name:
- 2-ethylhexan-1-ol
- Cas Number:
- 104-76-7
- Molecular formula:
- C8H18O
- IUPAC Name:
- 2-ethylhexan-1-ol
- Details on test material:
- - Source: Wako Pure Chemcials Industries, Osaka,, Japan
- Purity 2-EH: 98%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: bacteria. S. typhimurium TA98, TA100, TA1535, TA1537; TA1538; E. coli (WP2uvrA)
- Additional strain / cell type characteristics:
- other:
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 mix from polychlorinated biphenyl KC 500) induced male animals
- Test concentrations with justification for top dose:
- 0, 1, 5, 10, 50, 100, 500, 1000 µg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: experiments w/o S9 mix: AF-2 for TA100, TA 98, WP2uvrA; ENNG for TA 1535; 9AC for TA 1537, 4NQO for TA 1538; experiments with S9 mix: B(a)P for TA 100, TA 98, TA 1537 and TA 1538, 2AA for TA 1535 and WP2uvrA
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; preincubation;
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 hours
- Expression time (cells in growth medium): 48 hours
- Selection time (if incubation with a selection agent): 48 hours
- Fixation time (start of exposure up to fixation or harvest of cells): 48 hours
SELECTION AGENT (mutation assays): histidine or tryptophan deficiency
SPINDLE INHIBITOR (cytogenetic assays): not applicable (n..a.)
STAIN (for cytogenetic assays): n.a.
NUMBER OF REPLICATIONS: 2
NUMBER OF CELLS EVALUATED: n.a.
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: no data
OTHER EXAMINATIONS:
- Determination of polyploidy: no
- Determination of endoreplication: no - Evaluation criteria:
- no data
- Statistics:
- no data
Results and discussion
Test results
- Species / strain:
- other: S. typhimurioum and E.coli
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
ADDITIONAL INFORMATION ON CYTOTOXICITY: growth inhibition was noted in all test strains except TA1537 at 500 and 1000 µg/plate - Remarks on result:
- other: other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538; E. coli (WP2 uvrA)
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Except for TA 1537 toxicity was observed at doses of 500 and 1000 µg/plate in
all tester strains.
Mean number of revertants:
Dose (µg/plate) |
TA100 |
TA1535 |
E. coli WP uvrA |
TA98 |
||||
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
-S9 |
+S9 |
|
0 water |
149 |
161 |
28 |
15 |
32 |
33 |
29 |
39 |
0 DMSO |
150 |
154 |
30 |
15 |
30 |
34 |
32 |
42 |
1 |
144 |
170 |
39 |
23 |
32 |
31 |
24 |
44 |
5 |
166 |
171 |
23 |
19 |
30 |
26 |
33 |
61 |
10 |
161 |
149 |
26 |
18 |
27 |
31 |
29 |
48 |
50 |
155 |
147 |
33 |
13 |
26 |
33 |
28 |
57 |
100 |
133 |
151 |
19 |
14 |
28 |
33 |
37 |
51 |
500 |
0* |
0* |
0* |
0* |
0* |
0* |
0* |
0* |
1000 |
0* |
0* |
0* |
0* |
0* |
0* |
0* |
0* |
Positive control |
501 |
1084 |
1101 |
440 |
1082 |
359 |
278 |
809 |
Dose (µg/plate) |
TA1537 |
TA1538 |
||||||
-S9 |
+S9 |
-S9 |
+S9 |
|||||
0 water |
16 |
21 |
21 |
28 |
||||
0 DMSO |
18 |
22 |
22 |
28 |
||||
1 |
13 |
36 |
25 |
24 |
||||
5 |
11 |
28 |
33 |
30 |
||||
10 |
15 |
23 |
29 |
25 |
||||
50 |
16 |
30 |
25 |
30 |
||||
100 |
12 |
26 |
18 |
30 |
||||
500 |
12 |
39 |
0* |
0* |
||||
1000 |
16 |
28 |
0* |
0* |
||||
Positive control |
889 |
313 |
270 |
354 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
2-EH was not mutagenic in Salmonella typhimurium and E. coli with and without metabolic activation. - Executive summary:
The mutagenicity of 2 -EH was tested in bacterial test sytems (S. tyhimurium TA98, TA100, TA1535, TA1537, TA1538, and E. coli WP2 uvrA) according to OECD TG 471 and TG 472 both with and without metabolic activation in a dose range from 1 to 1000 µg/plate (Shimizu et al., 1985). 2 -EH did not increase the number of revertants in any of the test strains. Growth inhibition was seen at 500 and 1000 µg/plate. The negative and positive controls performed as expected. Therefore, 2 -EH was not mutagenic in baterial test systems in-vitro.
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