Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 418-570-8 | CAS number: 25383-07-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 18, 1994 - July 22, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/l
- Sampling method: sample removed at 24 hours from the treatment concentration. The sample was analysed for Fosfomycin PEA salt by Istituto G. Donegani. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test solution of Fosfomycin PEA salt were prepared by dissolving weighed amounts of test article directly to the study tanks in order to obtain the requested concentration.
- Differential loading: maximum loading was lower than 1.0 g fish/l (about 0.385 g/l)
- Controls: Yes, only water (0 mg/l) - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Source: "AQUA 1", Piazza Pistoni 10, 10015 IVREA (Torino, Italy)
- Length at study initiation (length definition, mean, range and SD): 3 cm (average value)
- Weight at study initiation (mean and range, SD): 0.385 g (average value)
ACCLIMATION
- Acclimation period: 2 weeks before start of the test.
- Type and amount of food: Mangime SERA
- Feeding frequency: daily until 24 hours before test
- Health during acclimation (any mortality observed): no mortality was recorded after 7 days of acclimation.
- Water source: municipal water main system
- Temperature: 23 ºC
- pH: 7.1
- Hardness: 39 mg/l CaCO3
- O2 concentration: 8.1 mg/l
- Parameter controls: immediately before the arrival of the fish and periodically (microbiologic count, heavy metals and other physico-chemical characteristics). - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 39 mg/l CaCO3
- Test temperature:
- 24-25 ºC
- pH:
- 6.56 - 7.14
- Dissolved oxygen:
- 8.0 - 8.2 mg/l
- Nominal and measured concentrations:
- Nominal concentrations: 0 (control) and 100 mg/l
Measured concentrations: 95.22 mg/l (24 h - nominal concentration of 100 mg/l) - Details on test conditions:
- TEST SYSTEM
- Test vessel: Tanks
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: Makrolon, 48x27x20 cm, 5 liters
- Aeration: No (to avoid foaming)
- Renewal rate of test solution (frequency/flow rate): Every 24 hours
- No. of organisms per vessel: 7 animals
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water main system
- Periodically, the water was analyzed for microbiologic count, heavy metals and other chemical and physical characteristics.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 hours light/dark daily.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
Observation of mortality: the fish were inspected after 24, 48, 72 and 96 hours. Fish were considered dead if touching of the caudal peduncle produced no reactions.
Observation of visible abnormalities: effects observed in the appearance, size and behaviour of the fish that make them clearly distinguishable from the control animals.
TEST CONCENTRATIONS
- Test concentrations: 0 (control) and 100 mg/l - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No animals of the control and treated groups showed visible signs of abnormalities.
- Observations on body length and weight: No animals of the control and treated groups showed visible signs of abnormalities.
- Other biological observations: No animals of the control and treated groups showed visible signs of abnormalities.
- Mortality of control: No animals of the control and the treated groups died
- Abnormal responses: No animals of the control and treated groups showed visible signs of abnormalities. - Sublethal observations / clinical signs:
Table 1. Acute toxicity study in fish (semi-static test). Parameter: dissolved oxygen – pH- Temperature.
Conc. 0 mg/L (control group)
Hours
Dissolved O2 (mg/L)
pH
T (ºC)
0
8.2
7.06
24
24*
8.0
7.00
25
24**
8.1
7.08
24
48*
8.0
7.00
24
48**
8.2
7.06
24
72*
8.0
7.00
24
72**
8.1
7.05
24
96
8.0
7.00
24
Conc. 100 mg/L (test item)
Hours
Dissolved O2 (mg/L)
pH
T (ºC)
0
8.2
6.49
24
24*
8.0
7.12
25
24**
8.1
6.57
25
48*
8.0
7.12
24
48**
8.2
6.56
24
72*
8.0
7.10
24
72**
8.1
6.56
24
96
8.0
7.14
24
* before water renewal; ** after water renewal.
- Validity criteria fulfilled:
- yes
- Remarks:
- No mortality was observed by the end of the test control, the conditions were constant during the test, the dissolved oxygen concentration was in the acceptable range, the substance concentration was maintained during the test.
- Conclusions:
- LC50 (based on mortality) of test material in Brachydanio rerio at 96 h exposure period was > 100 mg/l.
- Executive summary:
The acute toxicity of test material to the Brachydanio rerio, was determined in a 96 -hour, semi-static, aquatic (freshwater) effect test according to EU Method C.1. A total of 7 fish were exposed to 0 (control) and 100 mg/l nominal concentrations (limit test). The animals were treated and observed for 96 hours. No animals in the control group died or showed signs of visible abnormalities. No animals of the treated group died. The LC50 values at 24, 48, 72 and 96 hours were not calculated.
Reference
Description of key information
Key study: Test method according to EU Method C.1. GLP study.
LC50 (based on mortality) of test material in Brachydanio rerio at 96 h exposure period was > 100 mg/l.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Key study: Test method according to EU Method C.1. GLP study. LC50 (based on mortality) of test material in Brachydanio rerio at 96 h exposure period was > 100 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)