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Diss Factsheets
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EC number: 202-617-2 | CAS number: 97-90-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 999
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 002
- Reference Type:
- review article or handbook
- Title:
- Contact Sensitisation: Classification According to Potency.
- Author:
- ECETOC
- Year:
- 2 003
- Bibliographic source:
- Technical Report No. 87, European Centre for Ecotoxicology and Toxicology of Chemicals, Avenue Van Nieuwenhuyse 4, (Bte 6) B-1160 Brussels, Belgium, ISSN-0773-8072-87: 8
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 003
- Reference Type:
- review article or handbook
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- Version / remarks:
- now OECD 429 (LLNA local lymph node assay)
- Principles of method if other than guideline:
- Measurement of lymphocyte proliferative responses that are induced in draining lymph nodes following topical exposure of mice to test substance. An EC3 value is derived; this being the amount of a chemical sensitiser that is required to elicit a 3-fold increase in lymph node cell (LNC)
proliferative activity. - GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Ethylene dimethacrylate
- EC Number:
- 202-617-2
- EC Name:
- Ethylene dimethacrylate
- Cas Number:
- 97-90-5
- Molecular formula:
- C10H14O4
- IUPAC Name:
- 2-[(2-methylprop-2-enoyl)oxy]ethyl 2-methylprop-2-enoate
- Details on test material:
- Purity not specified, but commercial grade assumed.
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: 6 to 16 wk-old
- Weight at study initiation: no data
- Housing: no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum): no data
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
Study design: in vivo (LLNA)
- Vehicle:
- acetone/olive oil (4:1 v/v)
- No. of animals per dose:
- 4 or 5
- Details on study design:
- MAIN STUDY
ANIMAL ASSIGNMENT AND TREATMENT
- Name of test method: local lymph node assay (LLNA), standard protocol according to OECD 406
- Criteria used to consider a positive response:
A test material that, at one or more concentrations, causes an stimulation indices (SI) of 3 or greater is considered to have skin-sensitizing activity.
TREATMENT PREPARATION AND ADMINISTRATION:
Groups of mice (n = 4 or 5) are treated by topical a pplication, on the dorsum of both ears, with 25 µL of several concentrations of test material, or with an equal volume of the relevant vehicle alone. They are treated daily for 3 consecutive days, followed by a 2-day rest period before analysis. On the sixth day (5 days after initiation of treatment), the mice are injected intravenously, by the tail vein, with 250 µL of sterile phosphate-buffered saline (PBS) containing 20 µCi of [³H] methyl thymidine. Five hours later, the mice are killed.and the draining auricular lymph nodes are excised and pooled for each experimental group or for each individual animal. Single cell suspensions of lymph node cells are prepared. Lymph node cells are washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid (TCA) at 4°C.The samples, pelleted by centrifugation, are resuspended 12 to 18 hours later in 1 mL of 5% TCA and transferred to 10 mL of scintillation cocktail. lncorporation of tritium labeled thymidine [³H-TdR] is measured by beta-scintillation counting and expressed as disintegrations per minute (dpm). - Positive control substance(s):
- hexyl cinnamic aldehyde (CAS No 101-86-0)
- mercaptobenzothiazole (CAS No 149-30-4)
- other: benzocaine
Results and discussion
In vivo (LLNA)
Results
- Key result
- Parameter:
- EC3
- Value:
- 35
- Test group / Remarks:
- 4 or 5 animals
- Remarks on result:
- other: extremely weak sensitiser
Any other information on results incl. tables
EC3: 35 % extremely weak sensitiser
EC3 value is the estimated concentration of a chemical necessary to give a 3-fold stimulation of proliferation in lymph nodes compared to concurrent
vehicle-treated controls.
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (indication of skin sensitising potential) based on GHS criteria
- Conclusions:
- Ethyleneglycol dimethacrylate was found to be a extremely weak sensitiser in the LLNA test according to OECD 406 now OECD 429 (EC3: 35 % ).
- Executive summary:
In a dermal sensitization study (standard protocol) with Ethyleneglycol dimethacrylate (purity: no data) dissolved in acetone : olive oil (4 +1) as a vehicle, (4 or 5 animals per dose group) 6 to 16 weeks old female CBA/Ca mice were tested using the method of OECD 406 (now OECD 429 Local Lymph node Assay (LLNA)).
Groups of mice are treated by topical application, on the dorsum of both ears, with 25 µL of several concentrations of test material, or with an equal volume of the relevant vehicle alone. They are treated daily for 3 consecutive days, followed by a 2-day rest period before analysis. On the sixth day (5 days after initiation of treatment), the mice are injected intravenously, by the tail vein, with 250 µL of sterile phosphate-buffered saline (PBS) containing 20 µCi of [³H] methyl thymidine. Five hours later, the mice are killed.and the draining auricular lymph nodes are excised and pooled for each experimental group or for each individual animal. Single cell suspensions of lymph node cells are prepared. Lymph node cells are washed twice with an excess of PBS and precipitated with 5% trichloroacetic acid (TCA) at 4°C. The samples, pelleted by centrifugation, are resuspended 12 to 18 hours later in 1 mL of 5% TCA and transferred to 10 mL of scintillation cocktail. lncorporation of tritium labeled thymidine [³H-TdR] is measured by beta-scintillation counting and expressed as disintegrations per minute (dpm).
The validation-/positive control experiment was performed with alpha-Hexyl cinnamic aldehyde, 2 -mercaptobenzothiazole, and benzocaine.
In this study a Stimulation Indice (S.I.) of EC3: 35% was determined with the test item in acetone : olive oil (4 +1), respectively.
Therefore Ethyleneglycol dimethacrylate was found to be a extremely weak sensitiser in the LLNA test according to OECD 406.
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