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EC number: 247-465-8 | CAS number: 26115-70-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-05-24 to 2018-05-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 2010
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test System: Activated sludge
Origin: Municipal sewage treatment plant of 31137 Hildesheim, Germany
Reason for the selection: Non-adapted activated sludge from the sewage plant at Hildesheim is well suited as it receives predominantly municipal sewage and hardly any industrial chemical waste.
Pretreatment: The sludge was washed twice and was used within 24 h after sampling.
Date of receipt: 2018-05-24
Dry sludge concentration: 2.98 g/L, corresponding to 1.49 g/L in the test vessel for replicates without ATU and 2.94 g/L, corresponding to 1.47 g/L in the test vessel for replicates with ATU. - Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 3 h
- Test temperature:
- 18 - 22°C, actual: 20.0°C. The temperature was determined in the first replicate at test start with the pH-meter. The room temperature during the test was recorded continuously.
- pH:
- The pH value of the activated sludge was determined prior to test start. The pH value of the synthetic waste water was determined prior to use. Adjustment to 7.5 ± 0.5 was not necessary.
- Dissolved oxygen:
- After 3 h incubation, the oxygen concentration of each control, reference item and test item replicate was determined by means of oximeter every 5 seconds after transferring the solution to the measuring chamber and the oxygen depletion was recorded for at least 5 minutes with the software PreSens Measurement Studio 2
- Nominal and measured concentrations:
- 100 - 180 - 320 - 560 - 1000 mg/L were weighed out on bent object slides and were transferred into the test vessels.
- Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a (for total respiration) and N-Methylaniline (for nitrification)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 180 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 218 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of heterotrophic respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 88.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 805 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Details on results:
- The NOEC of 2,4,6-Tris[3-(trimethoxysilyl)propyl] isocyanurate (CAS 26115-70-8) for total respiration is 180 mg/L.
The EC10 for total respiration is 218 mg/L.
The NOEC for heterotrophic respiration is 1000 mg/L.
The NOEC for nitrification is 100 mg/L.
The EC10 for nitrification is 88.7 mg/L and the EC50 is 805 mg/L. - Validity criteria fulfilled:
- yes
- Conclusions:
- A 3 hour NOEC value of 180 mg/L was determined for the inhibition of total respiration of the substance using a relevant test method and in compliance with GLP. The result is considered to be reliable.
Reference
Biotic and abiotic factors in the definitive study
The pH-values of the activated sludge and synthetic waste water, the dry sludge concentration and the test temperature are documented in Table 6.
Table 6: Biotic and abiotic factors
pH-value of the activated sludge for test without ATU |
7.45 |
pH-value of the activated sludge for test with ATU |
7.30 |
pH-value of the synthetic waste water |
7.19 |
Dry solids concentration [g/L] of sludge for test without ATU |
2.98 |
Dry solid concentration [g/L] in test solution for test without ATU |
1.49 |
Dry solids concentration [g/L] of sludge for test with ATU |
2.94 |
Dry solid concentration [g/L] in test solution for test with ATU |
1.47 |
Test temperature [°C] |
20.0 |
Oxygen uptake rates, specific respiration rates and inhibition of the respiration
From the oxygen depletion the oxygen uptake rates were calculated in mg O2/L×h, additionally the spcific respiration rates based on the dry sludge concentration were calculated in mg O2/g×h. The inhibition of the oxygen uptake rates of the test and reference item concentrations in % was calculated in comparison to the oxygen uptake rates of the control. The results are listed in Table 7 to Table 15.
Table 7: Oxygen Uptake Rates and Specific Respiration Rates of the total Respiration of the Control
|
Repl. |
Oxygen |
Oxygen Uptake Rate R |
Specific Respiration Rate Rs |
|
|
[mg O2/L] |
[mg O2/(L×h)] |
[mg O2/g×h] |
Control |
1 |
7.85 |
30.8 |
20.7 |
2 |
7.60 |
32.0 |
21.5 |
|
3 |
6.92 |
29.0 |
19.5 |
|
4 |
7.78 |
28.3 |
19.0 |
|
5 |
7.69 |
28.6 |
19.2 |
|
6 |
7.44 |
26.8 |
18.0 |
|
|
Mean value |
29.3 |
19.7 |
|
Standard deviation |
1.7 |
|
||
CV [%] |
5.8 |
*) determined at start of the measurement
Repl. = Replicate ; CV = Coefficient of variation
Table 8: Oxygen Uptake Rates and Inhibition of the total Respiration of the Test Item Concentrations
Test Item Concentration |
Repl. |
Oxygen |
Oxygen Uptake Rate R |
Inhibition |
Mean Inhibition |
[mg/L] |
|
[mg O2/L] |
[mg O2/(L×h)] |
[%] |
[%] |
100 |
1 |
7.54 |
29.7 |
-1 |
2 |
2 |
7.36 |
29.8 |
-2 |
||
3 |
6.51 |
27.8 |
5 |
||
4** |
- |
- |
- |
||
5 |
7.00 |
27.7 |
5 |
||
180 |
1 |
7.58 |
27.7 |
5 |
6 |
2 |
7.86 |
28.3 |
3 |
||
3 |
7.25 |
25.7 |
12 |
||
4 |
8.11 |
28.5 |
3 |
||
5 |
7.26 |
26.6 |
9 |
||
320 |
1 |
7.90 |
24.0 |
18 |
18 |
2 |
7.53 |
25.0 |
15 |
||
3 |
7.42 |
23.5 |
20 |
||
4 |
8.03 |
25.1 |
14 |
||
5 |
7.26 |
22.7 |
23 |
||
560 |
1 |
8.11 |
22.5 |
23 |
28 |
2 |
7.91 |
22.5 |
23 |
||
3 |
7.40 |
21.3 |
27 |
||
4 |
8.14 |
22.7 |
23 |
||
5 |
7.71 |
16.9 |
42 |
||
1000 |
1 |
8.17 |
19.5 |
33 |
36 |
2 |
7.44 |
18.9 |
35 |
||
3 |
7.36 |
17.7 |
40 |
||
4 |
8.01 |
19.6 |
33 |
||
5 |
7.37 |
18.0 |
39 |
*) determined at start of the measurement
**) Replicate was broken while shaking overnight,not included in calculation
Repl. = Replicate
Table 9: Oxygen Uptake Rates of the heterotrophic Respiration of the Control Replicates with ATU
|
Repl. |
Oxygen |
Oxygen Uptake Rate R |
|
|
[mg O2/L] |
[mg O2/(L×h)] |
Control |
1 |
8.35 |
10.0 |
2 |
8.43 |
11.1 |
|
3 |
7.79 |
9.8 |
|
4 |
8.27 |
10.7 |
|
5 |
8.47 |
11.9 |
|
6 |
7.68 |
11.2 |
|
|
Mean value |
10.8 |
|
Standard deviation |
0.72 |
||
CV [%] |
6.67 |
*) determined at start of the measurement
Repl. = Replicate
CV = Coefficient of variation
Table 10: Oxygen Uptake Rates and Inhibition of the heterotrophic Respiration of the Test Item Concentrations
Test Item |
Repl. |
Oxygen [mg O2/L] |
Oxygen Uptake Rate R |
Inhibition |
Mean Inhibition |
100 + ATU |
1 |
8.20 |
15.5 |
-44 |
-18 |
2 |
8.48 |
12.8 |
-19 |
||
3 |
7.75 |
11.3 |
-5 |
||
4** |
8.33 |
12.2 |
-13 |
||
5 |
8.08 |
11.3 |
-5 |
||
180 + ATU |
1 |
8.39 |
13.8 |
-28 |
-27 |
2 |
8.23 |
16.3 |
-51 |
||
3 |
7.83 |
13.0 |
-20 |
||
4 |
7.98 |
12.9 |
-19 |
||
5 |
8.02 |
12.6 |
-17 |
||
320 + ATU |
1 |
8.13 |
13.8 |
-28 |
-21 |
2 |
8.34 |
14.4 |
-33 |
||
3 |
7.56 |
12.6 |
-17 |
||
4 |
8.19 |
13.0 |
-20 |
||
5 |
8.06 |
11.4 |
-6 |
||
560 + ATU |
1 |
8.28 |
10.1 |
6 |
3 |
2 |
8.23 |
11.3 |
-5 |
||
3 |
7.84 |
10.3 |
5 |
||
4 |
8.46 |
10.7 |
1 |
||
5 |
7.92 |
10.1 |
6 |
||
1000+ ATU |
1 |
8.32 |
10.7 |
1 |
6 |
2 |
8.25 |
10.8 |
0 |
||
3 |
7.54 |
10.4 |
4 |
||
4 |
8.11 |
10.2 |
6 |
||
5 |
7.94 |
8.8 |
19 |
*) determined at start of the measurement
**) Replicate without ATU was broken while shaking overnight,not included in calculation
Repl. = Replicate
Table 11: Calculated Oxygen Uptake Rates of the Nitrification of the Control
|
Repl. |
Oxygen Uptake Rate R |
|||
|
|
[mg O2/(L×h)] |
|||
Control |
1 |
20.8 |
|||
2 |
20.9 |
||||
3 |
19.2 |
||||
4 |
17.6 |
||||
5 |
16.7 |
||||
6 |
15.6 |
||||
Mean value |
18.5 |
||||
Standard deviation |
2.0 |
||||
CV [%] |
10.8 |
*) determined at start of the measurement
Repl. = Replicate
CV = Coefficient of variation
Table 12: Calculated Oxygen Uptake Rates and Inhibition of the Nitrification of the Test Item Concentrations
Test Item |
Repl. |
Oxygen Uptake Rate R |
Inhibition |
Mean Inhibition |
100 |
1 |
14.2 |
23 |
13 |
2 |
17.0 |
8 |
||
3 |
16.5 |
11 |
||
4* |
- |
- |
||
5 |
16.4 |
11 |
||
180 |
1 |
13.9 |
25 |
26 |
2 |
12.0 |
35 |
||
3 |
12.7 |
31 |
||
4 |
15.6 |
16 |
||
5 |
14.0 |
24 |
||
320 |
1 |
10.2 |
45 |
40 |
2 |
10.6 |
43 |
||
3 |
10.9 |
41 |
||
4 |
12.1 |
35 |
||
5 |
11.3 |
39 |
||
560 |
1 |
12.4 |
33 |
42 |
2 |
11.2 |
39 |
||
3 |
11.0 |
41 |
||
4 |
12.0 |
35 |
||
5 |
6.8 |
63 |
||
1000 |
1 |
8.8 |
52 |
54 |
2 |
8.1 |
56 |
||
3 |
7.3 |
61 |
||
4 |
9.4 |
49 |
||
5 |
9.2 |
50 |
Repl. = Replicate
*) Replicate (without ATU) was broken while shaking overnight, not included in calculation
EC Values:
The EC-values with confidence intervals are listed in Table 13.
Table 13: EC-Values with Confidence Interval of 2,4,6-Tris[3-(trimethoxysilyl)propyl] isocyanurate (CAS 26115-70-8)
|
Total Respiration |
Heterotrophic Respiration |
Nitrification |
|||
EC-values |
Confidence interval |
EC-values |
Confidence interval |
EC-values |
Confidence interval |
|
NOEC* |
180 |
– |
1000 |
– |
100 |
– |
EC10 |
218 |
170 – 270 |
¿ 1000 |
– |
88.7 |
¿100 -113 |
EC20 |
367 |
307 – 446 |
¿ 1000 |
– |
131 |
¿100 -178 |
EC50 |
¿ 1000 |
– |
¿ 1000 |
– |
805 |
494 - ¿1000 |
EC80 |
¿ 1000 |
– |
¿ 1000 |
– |
¿ 1000 |
– |
*) No statistically significant inhibition (P < 0.001), ANOVA, Dunnett`s Method
Reference test:
Total respiration
Table 14: Oxygen Uptake Rates, Inhibition of the total Respiration of the Reference Item Copper (II) sulphate pentahydrate Concentrations
Reference Item Concentration |
Repl. |
Oxygen |
Oxygen Uptake Rate R |
Inhibition |
Mean Inhibition |
[mg/L] |
|
[mg O2/L] |
[mg O2/(L×h)] |
[%] |
[%] |
58 |
1 |
8.08 |
22.0 |
25 |
28 |
2 |
8.27 |
21.1 |
28 |
||
3 |
7.71 |
19.8 |
32 |
||
100 |
1 |
8.28 |
13.7 |
53 |
53 |
2 |
8.31 |
14.7 |
50 |
||
3 |
7.89 |
12.7 |
57 |
||
180 |
1 |
8.67 |
5.1 |
83 |
82 |
2 |
8.63 |
5.5 |
81 |
||
3 |
7.87 |
5.2 |
82 |
*) determined at start of the measurement
Repl. = Replicate
In the reference test the EC50-value forcopper (II) sulphate pentahydrate was determined by linear regression with: EC50= 92.2 mg/L, 95% Confidence Interval = 87.9 – 96.5 mg/L
This is in the recommended range of validity of 53 - 155 mg/L.
Nitrification:
Table 15: Calculated Oxygen Uptake Rates, Inhibition of the Nitrification of the Reference Item N-Methylaniline Concentrations
Reference Item Concentration |
Repl. |
Oxygen Uptake Rate R |
Inhibition |
Mean Inhibition |
[mg/L] |
|
[mg O2/(L×h)] |
[%] |
[%] |
0.1 |
1 |
17.8 |
4 |
13 |
2 |
17.4 |
6 |
||
3 |
13.2 |
29 |
||
1.8 |
1 |
8.7 |
53 |
55 |
2 |
7.6 |
59 |
||
3 |
8.4 |
55 |
||
32 |
1 |
1.0 |
95 |
97 |
2 |
1.4 |
92 |
||
3 |
-0.7 |
104 |
*) determined at start of the measurement
Repl. = Replicate
In the reference test the EC50-value for N- Methylanilinewas determined by linear regression with: EC50= 1.25 mg/L, 95% Confidence Interval = 0.78 – 1.96 mg/L
Description of key information
Toxicity to microorganisms: 3 hour NOEC 180 mg/L (OECD 209; ASRI)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 180 mg/L
Additional information
An activated sludge respiration inhibition 3 hour NOEC (total respiration) of 180 mg/L and EC10 (total respiration) value of 218 mg/L were determined for the substance in a reliable study conducted according to an appropriate test protocol, and in compliance with GLP. The result is considered to be reliable.
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