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EC number: 207-300-2 | CAS number: 460-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from publication
Data source
Referenceopen allclose all
- Reference Type:
- other: authoritative database
- Title:
- Acute oral toxicity study in Rat.
- Author:
- U.S. National Library of Medicine
- Year:
- 2 017
- Bibliographic source:
- ChemIDplus
- Reference Type:
- other: Data is from authoritative database
- Title:
- Acute oral toxicity of test chemical in rat
- Author:
- IFA GESTIS
- Year:
- 2 018
- Bibliographic source:
- GESTIS Substance Database, 2018
- Reference Type:
- secondary source
- Title:
- Review of Toxicological Literature
- Author:
- National Toxicology Program (NTP)
- Year:
- 2 005
- Bibliographic source:
- National Toxicology Program (NTP), National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health, U.S Department of Health and Human Services, January 2005
- Reference Type:
- secondary source
- Title:
- Initial submission: Median lethal dose toxicity study with test chemical in Rats with cover letter
- Author:
- NTRL
- Year:
- 1 986
- Bibliographic source:
- NTRL report, 0TSO540497- 10/15/1986
- Reference Type:
- other: Data is from authoritative database
- Title:
- Acute oral toxicity of test chemical in rats
- Author:
- RTECS
- Year:
- 2 018
- Bibliographic source:
- RTECS (registry of toxic effect of chemical substance data base), 2018
- Reference Type:
- publication
- Title:
- : Provisional Peer-Reviewed Toxicity Values for test chemical
- Author:
- U.S. Environmental Protection Agency (USEPA) National Center for Environmental Assessment (NCEA) Office of Research and Development (ORD)
- Year:
- 2 017
- Bibliographic source:
- Provisional Peer-Reviewed Toxicity Values for test chemical, EPA/690/R-17/005,09-25-2017
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Principles of method if other than guideline:
- Acute oral toxicity study of test chemical in rats
- GLP compliance:
- not specified
- Test type:
- other: Single dose acute oral toxicty
- Limit test:
- no
Test material
- Reference substance name:
- 1-bromo-4-fluorobenzene
- EC Number:
- 207-300-2
- EC Name:
- 1-bromo-4-fluorobenzene
- Cas Number:
- 460-00-4
- Molecular formula:
- C6H4BrF
- IUPAC Name:
- 1-bromo-4-fluorobenzene
- Reference substance name:
- 4-Bromofluorobenzene
- IUPAC Name:
- 4-Bromofluorobenzene
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material :4-Bromofluorobenzene (4-BFB)
- Molecular formula :C6H4BrF
- Molecular weight :175.0 g/mole
- Substance type:Organic
- Physical state:Clear coloureless to slightly yellow liquid
Purity98 %
- Impurities (identity and concentrations):2 %
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Kingston, New york
- Age at study initiation: 8 weeks old
- Housing: Animals were housed singly in suspended, stainless steel, wire –mash cages with dimensions of 8” X 14” X 8” (wXdXh). Each rat was assigned a unique identification number which was recorded on a card affixed to the cage.
- Diet (e.g. ad libitum): Purina certified rodent chow #5002, ad libitum
- Water (e.g. ad libitum): water, ad libitum
- Acclimation period: 8 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 ± 2 °C
- Humidity (%): 50 ± 10%
- Photoperiod (hrs dark / hrs light): Timer-controlled, 12 hrs dark / 12 hrs light
IN-LIFE DATES: From: 3/20/85
To: 5/22/85
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 1000, 2000, 3000 and 5000 mg/kg bw
- Justification for choice of vehicle: Marola corn oil
DOSAGE PREPARATION (if unusual): 4-Bromofluorobenzene (4-BFB) as emulsion in Marola corn oil. - Doses:
- 1000, 2000, 3000 and 5000 mg/kg bw
- No. of animals per sex per dose:
- Total : 40
1000 mg/kg bw: 10 male
2000 mg/kg bw: 10 male
3000 mg/kg bw: 10 male
5000 mg/kg bw: 10 male - Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Daily
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs and body weight were examined. - Statistics:
- LD50 values were calculated from the mortality data using method of 0.0.Finney.
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 700 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 2 200 - < 3 200
- Remarks on result:
- other: No effect on survival, clinical and body weight
- Mortality:
- At 3000 mg/kg and 5000 mg/kg bw, 7 and 10 male rats were dead, 1 at 2000 mg/kg bw and no effect on survival of 1000 mg/kg bw treated rats were observed.
- Clinical signs:
- other: Tremor, Limpness change in motor activity, clear discharge from eyes and ataxia were observed in treated rats.
- Gross pathology:
- No data available
- Other findings:
- No data available
Any other information on results incl. tables
Sr no. |
Dose rate |
Average body weight |
Emulsion concentration (mg/ml) |
Average dose |
Mortality ratio |
1 |
1000 |
231(227-243) |
225 |
1.0 |
0/10 |
2 |
2000 |
251(235-268) |
300 |
1.7 |
1/10 |
3 |
3000 |
242(226-253) |
300 |
2.4 |
7/10 |
4 |
5000 |
259(255-267) |
300 |
4.3 |
10/10 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified
- Conclusions:
- LD50 was considered to be 2700 mg/kg bw (2200 – 3200 mg/kg ) when Crl:CD (SD)BR male rats were treated with test chemical orally by gavage.
- Executive summary:
In a acute oral toxicity study,Crl:CD (SD)BR male rats were treated with test chemical in the concentration of 1000, 2000, 3000 and 5000 mg/kg bw as emulsion in Marola corn oil orally by gavage and observed for 14 days. 7 and 10 male rats were dead at 3000 mg/kg and 5000 mg/kg bw, 1 at 2000 mg/kg bw and no effect on survival of 1000 mg/kg bw treated rats were observed. Tremor, Limpness change in motor activity, clear discharge from eyes and ataxia were observed in treated rats. Slight to sever decrease in body weight were observed in treated rats. Therefore,LD50 was considered to be 2700 mg/kg bw(2200 – 3200 mg/kg ) whenCrl:CD (SD)BR male rats were treated with test chemical orally by gavage.
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