Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 287-494-3 | CAS number: 85536-14-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-10-31 to 1989-11-2
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Followed guideline with minor deviation, well documented
- Justification for type of information:
- Category Approach; test material is C12 LAS, a major component of reference substance LAS. LAS provides suitable read across for LAB Sulfonic Acids as both form the identical chemical species in aqueous solutions at neutral (environmental) pH, namely, the LAS ion (C10-13 linear alkyl benzene-SO3-) and would be expected to have similar ecotoxicological properties.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- Measurement of test concentration is not available.
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- EC Number:
- 270-115-0
- EC Name:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts
- Cas Number:
- 68411-30-3
- Molecular formula:
- Not applicable for UVCB
- IUPAC Name:
- sodium 4-undecylbenzenesulfonate
- Details on test material:
- - Name of test material (as cited in study report): Linear alkyl benzene sulfonate (LAS ex Petresa) (E-3474.01)
- Molecular formula (if other than submission substance): Molecular formula was not given but structure is given in the report
- Molecular weight (if other than submission substance): Not available
- Structural formula attached as image file (if other than submission substance): Structure provided in the study report
- Substance type: Pure active substance
- Physical state: Liquid
- Radiochemical purity (if radiolabelling): Not applicable
- Specific activity (if radiolabelling): Not applicable
- Locations of the label (if radiolabelling): Not applicable
- Expiration date of radiochemical substance (if radiolabelling): Not applicable
- Stability under test conditions: Not available
- Storage condition of test material: Cooled conditions under 4-8 degree C
- Other: None
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples for analysis of each concentration tested were taken at 0, 24 and 48 h
- Sampling method: The samples were taken in glass bottles and preserved by adding 1ml of 37% formalin in 99 ml of test sample solution
- Sample storage conditions before analysis: Not available
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 200 mg of Linear alkyl benzene sulfonate (LAS ex Petresa) (E-3474.01) (ETS311) was accurately weighed out and dissolved in 2000 ml of dilution water, resulting in a solution containing 100mg/L. From this solution 32, 56, 100, 180, 320 and 560 ml were taken and diluted with dilution water to one liter. These solutions, containing 3.2, 5.6,10, 18, 32 and 56 mg/L and the solution containing 100mg/L were used as test solutions; portions of 100ml of each test solution were placed in each of four 150 ml beakers.
- Eluate: Not applicable
- Differential loading: Not applicable
- Controls: Control vessel contained 100% dilution water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Not applicable
- Concentration of vehicle in test medium (stock solution and final test solution): Not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: Daphnia magna Straus 1820
- Source: TNO Nutrition and Food Research Institute Laboratory culture
- Age at study initiation (mean and range, SD): <24 h old
- Weight at study initiation (mean and range, SD): Not available
- Length at study initiation (length definition, mean, range and SD): Not available
- Valve height at study initiation, for shell deposition study (mean and range, SD): Not applicable
- Peripheral shell growth removed prior to test initiation: Not applicable
- Method of breeding: Organisms were cultured in laboratory kept under conditions specified in NPR 6503
- Feeding during test: Not fed
- Food type: Not applicable
- Amount: Not applicable
- Frequency: Not applicable
ACCLIMATION
- Acclimation period: Not available
- Acclimation conditions (same as test or not): Not available
- Type and amount of food: Not available
- Feeding frequency: Not available
- Health during acclimation (any mortality observed): Not available
QUARANTINE (wild caught)
- Duration: Not applicable
- Health/mortality: Not applicable
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- The hardness of the test solution was 215 mg/L as CaCO3
- Test temperature:
- Temperature was maintained at 20 +/-1 degree C
- pH:
- pH was measured at 0 and 48 h. Overall pH ranged 7.9-8.1. See the table 1 given below
Control, 3.2, 5.6, 10, 18, 32, 56 mg/L pH ranged as 8-8.1
100 mg/L: range 7.9-8 - Dissolved oxygen:
- DO was measured at 0 and 48 h. Overall DO ranged 8.8-9.6 mg/L. See the Table 2 given below.
Control: range 8.8-9.5 mg/L
3.2 mg/L: range 8.8-9.3 mg/L
5.6 mg/L: range 8.7-9.1 mg/L
10 mg/L: range 8.8-9.4 mg/L
18 mg/L: range 8.8-9.3 mg/L
32 mg/L and 56 mg/L: range 8.8-9.2 mg/L
100 mg/L: range 8.8-9.6 mg/L - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal test concentrations based on the test material as received were 0, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L. The test material contained 12.09% active. Samples were taken for analytical measurement of test concentrations, but those results are not available in this study report.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed: Not available
- Material, size, headspace, fill volume: 150 ml all-glass beakers, containing 100 ml test solution.
- Aeration: Not aerated
- Type of flow-through (e.g. peristaltic or proportional diluter): Not applicable
- Renewal rate of test solution (frequency/flow rate): Not renewed
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): Not applicable
- Biomass loading rate: 100 ml of test solution/5 organisms=20 ml test solution per organism
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The dilution water was prepared by the addition of several salts to groundwater from a locality near Linschoten (The Netherlands). The composition of the dilution water (DSWL) was Na+: 1.20 mmol/L, K+: 0.23 mmol/L, Cl-: 2.59 mmol/L, SO4:2-: 0.67 mmol/L, HCO3-: 1.39mmol/L and contained several other trace elements (<<1mg/L). Water had hardness of 215 mg/L as CaCO3, pH of 8-8.2. This DSWL had proven to be suitable for the culture of the test organism used in this test.
- Total organic carbon: Not available
- Particulate matter: Not available
- Metals: Not available
- Pesticides: Not available
- Chlorine: Not available
- Alkalinity: Not available
- Ca/mg ratio: 1.37 mmol/L Ca 2+/ 0.78mmol/L Mg+2
- Conductivity: Not available
- Culture medium different from test medium: Not specified in the study report.
- Intervals of water quality measurement: pH and DO were measured at the beginning and at the end of the test. Temperature was maintained constant throughout the test.
OTHER TEST CONDITIONS
- Adjustment of pH: Not applicable
- Photoperiod: 16 h light and 8 h dark
- Light intensity: Not available
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The immobile animals were counted after 24 h and 48 h. At the same time, the condition (Swimming behaviour, color or any other observable morphological or behavioural criterium) of the mobile animals was visually compared with that of the control animals (blanks)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Justification for using less concentrations than requested by guideline: Not applicable
- Range finding study: No
- Test concentrations: N/A
- Results used to determine the conditions for the definitive study: N/A - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 2.5-3.5 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 5.4-7.5 mg/L
- Details on results:
- - Behavioural abnormalities: At some test concentrations, the daphnids moved irregularly and more slowly than controls (see Table 3 below for details).
- Observations on body length and weight: None
- Other biological observations: At some test concentrations, some daphnids were paler than the controls (see Table 3 below for details).
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: None - Results with reference substance (positive control):
- Not applicable
- Reported statistics and error estimates:
- The EC50 values and their confidence intervals were calculated by means of a parametric model developed by a Kooijiman.
Any other information on results incl. tables
Table 3: Number of mobile animals and their condition at several concentrations of E-3474.01 (ETS 311) and after several exposure times(a, b, cand d are quadruplicate test vessels).
Time (h) |
Concentration of test substance (mg.l"1) |
|||||||||||||||
0 |
3.2 |
5.6 |
10 |
|||||||||||||
a |
b |
c |
d |
a |
b |
c |
d |
a |
b |
c |
d |
a |
b |
c |
d |
|
0 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
24 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
48 |
51) |
51) |
51) |
51) |
52) |
52) |
52) |
52) |
52) |
52) |
52) |
53)4) |
43)4) |
53)4) |
53)4) |
53)4) |
Time (h) |
Concentration of test substance (mg.l'1) |
|||||||||||||||
18 |
32 |
56 |
100 |
|||||||||||||
a |
b |
c |
d |
a |
b |
c |
d |
a |
b |
c |
d |
a |
b |
c |
d |
|
0 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
24 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
1 |
3 |
3 |
3 |
0 |
0 |
0 |
0 |
48 |
43)4) |
43)4) |
33)4) |
33)4) |
13)4)5) |
23)4)5) |
33)4)5) |
23)4)5) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1) Condition of all test animals, visually assessed, normal (= good),
2) Condition of all test animals, visually assessed, equal to that of the control animals.
3) The quoted number animals were mobile, according to the definition given in OECD Guideline no. 202, but their condition (see section 2.4) was not equal to that of the control animals; they moved irregularly and more slowly than the controls.
4) The quoted number of mobile animals was paler than the control animals.
5) The animals were almost immobile
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- All criteria of OECD 202 were met
- Conclusions:
- The 48-hour EC50 of Linear alkyl benzene sulfonate (LAS ex Petresa) to Daphnia magna was 2.9 mg/L based on nominal concentration of the active ingredient.
- Executive summary:
In a 48 h acute toxicity study, Daphnia magna were exposed to Linear alkyl benzene sulfonate (LAS ex Petresa) at nominal concentrations of 0 (Control), 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L under static condition, based on the sample as received. The sample was 12.1% active ingredient, and results were adjusted to the active ingredient basis.
The 48 h EC50 value based on mobility was 2.9 mg a.i./L with 95% CL of 2.5 to 3.5 mg/L. This toxicity study is classified as acceptable and satisfies the guideline requirement for the current OECD 202 Daphnia acute toxicity study
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)