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EC number: 682-131-9 | CAS number: 1190427-50-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-06-14 to 2016-10-20
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- slowly decomposition in water
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the test (0h), the control, and following test solutions: 6.25; 25, and 100 mg/l were analyzed before division to the test vessels, each group: 4 samples of 10 ml
After 24 h exposure, all replicates of control and of all five test concentrations were analyzed, each group: 4 samples of 10 ml
At the end of the test (48h), all replicates of of the control, and following test solutions 6.25; 25, and 100 mg/l were analyzed before division to the test vessels, each group: 4 samples of 10 ml
The samples were filtered (0.45µm syring filter), filled in plastic centrifuge tubes and stored (<8weeks) in the fridge (5°C +-3°C). Of each treatement, one of the samples from 0h; 24 h; and 48 h was sent to the analytical laboratory and analyzed. The remaining samples were stored as retain samples in the fridge until finalization of the study - Vehicle:
- no
- Details on test solutions:
- The stock solution was prepared by adding 99.9 mg test item to 1000 ml ISO medium and shaking for 24 h using overhead shaker at 24.2-25.1 °C in the dark. As the test substance is a very poorly soluble solid, the stock solution was filtered through a fibre glass filter with retaining range till 0.6 µm. The filter was prepared by rinsing with purified water and preconditioning with ca 250 ml stock solution (which was discarded), to reduce adsorption of the test item. The negative control (ISO-medium) was treated in the same way as the test substance solution. Into each test vessel, 40ml test substance solution and ISO medium was transferred,
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Daphnia magna Straus
- Source: Federal Environment Agency Berlin
- Feeding during test: no
- Food type: Desmodesmus subspicatus
- Amount: 0.1 mg C/daphnid/day - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- ISO 6341 medium were used
- Test temperature:
- 19.8-21.4 °C
- pH:
- control: 7.0-7.8
test solution: 7.5-7.9 - Dissolved oxygen:
- control: 7.3-7.9 mg/l
test solution: 7.3-7.7 mg/l - Salinity:
- not applicable
- Nominal and measured concentrations:
- nomial loading rates: o; 6.25; 12.5; 25; 50, and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers 50 ml
- Type (delete if not applicable): open
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO 6341 medium without EDTA
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16h light; 8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : swimming capability was determined after 24 h and 48 h exposure, The pH as well as O2 concentrations were measured at the start (ßh) and at the end of the test (48h), temperature was measuered continuously
RANGE-FINDING STUDY
- Test concentrations: preliminary test without GLP, nominal loading rates of 10, 40, 160 mg/l of not filtered test material
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- 19.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- 12.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 6.25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Other biological observations: no
- Mortality of control: no
- Other adverse effects control: no
- The measured Ru-concentrations in the test substance treatments were below the limit of quantification (< 1µg/l) up to 1.6 µg/l. The Ru-concentrations in the control were below the limit of quantification - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50(24h) = 1.30 mg/l ; EC50(48h) = 0.82 mg/l - Reported statistics and error estimates:
- statistical evaluation with ToxRat Professional 3.2.1
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test substance is classified as harmful to aquatic life with long lasting effects.
- Executive summary:
A valid static Daphnia magna acute immobilisation test with the test substance according to OECD Guideline under GLP was performed. The EL50 loading rate of 19.2 mg/l after 48 hours was determined
Reference
Table 1: Immobility after 24 h and 48 h exposure
Nominal test item Loading rate [mg/l] |
Mobile Daphnia (replicates 1-4) |
Immobility [%] |
||||||||||||
0 h |
24 h |
48 h |
24 h |
48 h |
||||||||||
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
1 |
2 |
3 |
4 |
|||
Control |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
0 |
0 |
6.25 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
4 |
5 |
0 |
5 |
12.5 |
5 |
5 |
5 |
5 |
5 |
4 |
5 |
4 |
3 |
5 |
4 |
3 |
10 |
25 |
25 |
5 |
5 |
5 |
5 |
2 |
3 |
4 |
1 |
2 |
3 |
3 |
1 |
50 |
55 |
50 |
5 |
5 |
5 |
5 |
0 |
1 |
0 |
1 |
0 |
0 |
0 |
0 |
90 |
100 |
100 |
5 |
5 |
5 |
5 |
|
0 |
0 |
0 |
0 |
0 |
0 |
0 |
100 |
100 |
Description of key information
The test substance is classified as harmful to aquatic life with long lasting effects.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 19.2 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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