Registration Dossier
Registration Dossier
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EC number: 215-863-0 | CAS number: 1435-71-8
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-[(o-nitrophenyl)azo]-p-cresol
- EC Number:
- 215-863-0
- EC Name:
- 2-[(o-nitrophenyl)azo]-p-cresol
- Cas Number:
- 1435-71-8
- Molecular formula:
- C13H11N3O3
- IUPAC Name:
- 4-methyl-2-[(2-nitrophenyl)diazenyl]phenol
- Details on test material:
- - Name of test material (as cited in study report): TKA 40136, Monoazo 3113
- Physical state: Red-brownish solid
- Analytical purity: > 95%
- Lot/batch No.: Pa 369
- Storage condition of test material: At room temperature in the dark
- Expiry date: 1997-11-1
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Laboratory wastewater plant treating municipal and synthetic sewage
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 25 mg/L
- Based on:
- test mat.
- Initial conc.:
- ca. 15 mg/L
- Based on:
- other: TOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2 °C
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: A mixture of oxygen and nitrogen was led through a botlle, containing 0.5-1 L 0.0125 M Ba(OH)2 solution to trap CO2 which might be present in small amounts. The CO2-free air was sparged through the scrubbing solutions at a constant rate.
- Measuring: Carbon dioxide trap (Barium hydroxide), titration with 0.05 M HCl
- Test performed in open system: no
SAMPLING
- Sampling frequency: Every second or third day up to day 10, thereafter at least every fifth day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Toxicity control
Reference substance
- Reference substance:
- other: 40 mg/L Sodium acetate
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 7.3 - <= 9.7
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 30.2% after 5 days, 89.1% after 14 days and > 100% after 28 days
Applicant's summary and conclusion
- Interpretation of results:
- other: Not readily biodegradable under this test conditions
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