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EC number: 418-570-8 | CAS number: 25383-07-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 28, 1994 - August 25, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Effluent from a strongly aerated laboratory activated sludge plant (RBM plant)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Effluent from a strongly aerated laboratory activated sludge plant (RBM plant).
- Storage conditions: The effluent was kept under aerobic conditions in the period between sampling and application.
- Preparation of inoculum for exposure: The sample was filtered through a coarse filter, the first 200 ml being discarded. The rest of the filtrate was kept aerobic until used. The inoculum was used on the day of collection.
- Initial cell/biomass concentration: Number of bacteria was determined by means of Cult-Dip plus (Merck)
- Water filtered: yes
- Type and size of filter used, if any: coarse filter. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Mineral medium:
Deionized water: <0.01 mg/Cu/l (EEC Directive 80/778)
Salt stock solutions:
Solution 1 (buffer solution): 8.5g KH2PO4, 28.5g K2HPO·3H2O, 33.4g Na2HPO4·2H2O and 0.5g NH4Cl were dissolved in 1 L distilled water. pH=7.4
Solution 2: 22.5 g MgSO4·7H2O/L distilled water
Solution 3: 36.4 g CaCl2·2H2O distilled water
Solution 4: 0.25 g FeCl3·6H2O distilled water
Preparation of the mineral medium: 1 ml each solutions 1 to 4 was added to 800 ml up to 1 L.
The large bottles were first filled to about one third of their volume with fully aerated mineral medium by hose. 0,5 ml of test or/and reference substance were added (0,5 ml of 1% w/v water solution) to obtain 2 mg/l concentration. Subsequently, the inoculum was added to the solution. Each prepared solution was filled into the respective group of bottles by hose from the lower quarter of the bottle to ensure adequate mixing.
- Test temperature: 20 ºC
- pH adjusted: No
- Aeration of dilution water: Yes, the inoculum was kept aerobic until used (shaking).
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Calibrated BOD bottles with glass stoppers.
- Number of culture flasks/concentration: 2 series of bottles (9 bottles): 1 (zero control), 2 (7 days), 2 (14 days), 2 (21 days), 2 (28 days).
- Method used to create aerobic conditions: The inoculum was kept aerobic until used (shaking). The inoculum was used on the day of collection.
- Measuring equipment: The concentration of dissolved oxygen was determined with the Winkler method.
- Other: water bath for keeping the bottles at constant temperature (20 ºC) excluding light.
SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: The bottles were removed from the water bath (20 ºC, dark) after 7, 14, 21 and 28 days and analysed for dissolver oxygen determination.
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes.
- Toxicity control: Yes, inhibition test (reference substance and test control) - Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- The test article Fosfomycin PEA salt did not show biodegradability.
The test article Fosfomycin PEA salt did not show any biochemical oxygen demand during the test period.
The test article has no inhibitory effects on the bacteria inoculum at the concentration used in this study. - Key result
- Parameter:
- COD
- Value:
- 1.638 mg O2/g test mat.
- Results with reference substance:
- The biodegradability of the reference substance (sodium acetate) on days 7, 14, 21 and 28 days was 71, 73, 83 and 92% respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- Oxygen depletion in blank test did not exceed 1.5 mg dissolved 02/l after 28 d. Oxygen concentration in test bottle did not fall below 0,5 mg/L.
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The average COD value was 1.638 mg O2/mg.
In the conditions adopted in this study, the test article Fosfomycin PEA salt was not biodegradable and showed no inhibitory effect (toxicity) on the bacteria inoculum at the concentration used (2mg/l), although the biodegradation value in the inhibition test (about 37% of ThOD) may be considered as borderline. - Executive summary:
The ready biodegradability of the test article Fosfomycin PEA salt in an aerobic aqueous medium at the concentration of 2 mg/l was performed according to EU Method C.4 and OECD Guideline 301D by the Closed Bottle Test. Parallel groups of BOD bottles were prepared for the determination of the BOD values of the test article (2 mg/l of Fosfomycin PEA salt), reference substance (2 mg/l of sodium acetate, to check the activity of the substance) and the test plus the reference substance (inhibition test, to check inhibition effects of the substance, if any) together with the blank, with inoculum, in two simultaneous experimental series. The degradation was followed by oxygen analysis (Winkler method) over a 28 day period (7, 14, 21 and 28 days). The test article Fosfomycin PEA salt did not show any biochemical oxygen demand during the test period.
The biodegradability was calculated as BOD (mgO2/mg) / COD (mgO2/mg) x 100.
The COD test was performed in accordance with ISO 606 -1986. The average COD of the test article was 1.638 mg O2/mg.
In the condition adopted in this study the test article Fosfomycin PEA salt was not biodegradable and showed no inhibitory effect (toxicity) on the bacteria inoculum at the concentration used (2 mg/l), although the biodegradation value in the inhibition test (about 37% of ThOD) may be considered as borderline.
Reference
The test article Fosfomycin PEA salt did not show biodegradability.
The test article Fosfomycin PEA salt did not show any biochemical oxygen demand during the test period.
The test article has no inhibitory effects on the bacteria inoculum at the concentration used in this study.
Description of key information
Key study: Test method according to EU Method C.4 and OECD Guideline 301D. GLP study.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Key study: Test method according to EU Method C.4 and OECD Guideline 301D. GLP study. In the conditions adopted in the study, the test article Fosfomycin PEA salt was not biodegradable at the concentration of 2 mg/l.
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