3-nitro-o-xylene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Principles of method if other than guideline:

other: according to the method described by Könemann (1981).
The 14 day LC50 values of the various compounds were determined following the
procedure outlined by Koenemann (1981), with two small changes: concentrations
were increased in geometrical progression with a factor of 2, and ten guppies were
exposed to each concentration.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3-nitro-o-xylene

EC Number:

201-474-3

EC Name:

3-nitro-o-xylene

Cas Number:

83-41-0

Molecular formula:

C8H9NO2

IUPAC Name:

1,2-dimethyl-3-nitrobenzene

Test material form:

liquid

Details on test material:

3-Nitro-o-xylene
pure (not specified)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- water samples were taken at least on four days during the experimental period, both before and after renewal of the solutions

Test solutions

Details on test solutions:

- concentrations found after 24 h corresponded to at least 80% of the amount added

Test organisms

Test organisms (species):

Poecilia reticulata

Details on test organisms:

- fish used for the toxicity experiments were male and female
- fish were reared in own laboratory (Department of Veterinary Pharmacology, Pharmacy and Toxicology, University of Utrecht, Utrecht, NL)
- age of fish varied from 2-3 months at the start of the experiments

Study design

Test type:

other: not specifed

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Test conditions

Hardness:

25 mg/L as CaCO3

Test temperature:

21 - 23 °C

pH:

6.8 - 7.2

Dissolved oxygen:

> 4.5 mg/L

Details on test conditions:

- all fish were acclimatized for at least 12 days prior to the experiments.
- all fish were fed a commercial fishfood daily.
- all experiments were carried out in standard water (SW), prepared according to Alabaster and Abram (1964).

Results and discussion

Effect concentrations

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

6.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)