6,10,14-trimethylpentadecan-2-one

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10,14-trimethylpentadecan-2-one

EC Number:

207-950-7

EC Name:

6,10,14-trimethylpentadecan-2-one

Cas Number:

502-69-2

Molecular formula:

C18H36O

IUPAC Name:

6,10,14-trimethylpentadecan-2-one

Details on test material:

- Name of test material (as cited in study report): Hexahydrofarnesylaceton
- Analytical purity: 93.4 %
- Physical state: fluid
- Lot/batch No.: 22 .04 .88

I

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Remarks:

Cremophor RH 40

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (100 mg/l test substance) was stirred for 1020 min at 20 °C. Cremophor RH 40 was added. A serial dilution was prepared.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Cremophor RH 40
- Concentration of vehicle in test medium (stock solution and final test solution): 100 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Daphnia magna Straus, originally obtained from the Institute National de Recherche Chimique Appliquee, France and cultured in the laboratories of BASF since 1978.
- Age at study initiation (mean and range, SD): 2 - 26 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.7 +/- 0.5 mmol/l

Test temperature:

20 °C

pH:

7.7 - 8.0

Dissolved oxygen:

8.1 - 9.5 mg/l

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.0195,
0.039,
0.078,
0.156,
0.313,
0.625,
1.25,
2.5,
5,
10,
control,
solvent control

Details on test conditions:

TEST SYSTEM
- Test vessel: flat bottom glass tubes
- Fill volume: 10 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater, prepared using a dionised water (Conductivity: < 0.05 µS/cm)
- Ca/mg ratio: 4:1
- Conductivity: 600 - 700 µS/cm


OTHER TEST CONDITIONS
- Photoperiod: continuous darkness


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mobility after 0, 3, 6, 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.078 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.281 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

All Daphnia in test concentrations 2.5, 5, 10 mg/1 swam at the water surface.

Reported statistics and error estimates:

Probit analysis