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Diss Factsheets
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EC number: 203-744-6 | CAS number: 110-18-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 23 September to 23 October 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The study was performed to determine the potential for Bulab 600 to produce toxicity from a single 1-hour inhalation exposure.
- GLP compliance:
- no
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- N,N,N',N'-tetramethylethylenediamine
- EC Number:
- 203-744-6
- EC Name:
- N,N,N',N'-tetramethylethylenediamine
- Cas Number:
- 110-18-9
- Molecular formula:
- C6H16N2
- IUPAC Name:
- [2-(dimethylamino)ethyl]dimethylamine
- Test material form:
- liquid
- Details on test material:
- clear, colourless to pale yellow
Constituent 1
- Specific details on test material used for the study:
- Bulab 600
Lot 0-5201
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- Source: Charles River Breeding Laboratories
Housing: Individually housed
Temperature controlled
Food: Purina rat chow, ad libitum except during exposure
Water: Madison city well water, ad libitum except during exposure
Age at experimental start: 8 weeks
Weight at experimental start: 158-240 g
Acclimatisation period: 7 days
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Remark on MMAD/GSD:
- Particle size not measured.
- Details on inhalation exposure:
- The initial screen level was conducted at a nominal concentration of 69100 mg/m^3 of air for 1 hour and the second level at 17900 mg/m^3 of air for 1 hour. The time for equilibration (90% of desired concentration) was 14 minutes for 69.1 mg/L of air and 8 minutes for 17.9 mg/L of air thereby the exposure time was 74 and 68 minutes, respectively. Administration of the test material was during only the initial 60 minutes. The test exposures were conducted with samples of the test material in which each contained the same concentration of formulated ingredients. The test material was administered directly into the chamber using the Spraying Systems unit which was equilibrated prior to conducting the exposure by running the unit and calculating the dispersion rate several times. The unit was checked for variation in the rate of dispersion periodically during the exposure period. Exposures were conducted in an 842 L stainless steel semi-portable exposure chamber which was equipped with an exhaust port which was connected to an exhaust fan, providing an adjustable air flow through the chamber. Air flow was determined by a calibrated pressure gauge which measured the pressure drop across a defined inlet orifice. The test material was administered into the chamber near the junction for the air inlet pot, which allows the test material and incoming air to mix evenly within the chamber at the top before being drawn down over the animals.
- Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- 1 h
- Remarks on duration:
- Equilibration time was 14 minutes for high dose and 8 minutes for low dose
- Concentrations:
- 17900 and 69100 mg/m^3 of air for 1 hour, equivalent to 17.9 and 69.1 mg/L of air
- No. of animals per sex per dose:
- Five
- Control animals:
- yes
- Details on study design:
- Animal observations were recorded periodically during exposure. Immediately following and up to 1 hour after exposure to the compound, the animals were observed for pharmacotoxic signs and mortality. Thereafter, the animals were observed daily for 14 days for pharmacotoxic effects and mortality. Body weights for all animals were taken just prior to compound exposure and again at 7 and 14 days post exposure for survivors. Animals were sacrificed at 14 days. They were subjected to a gross necropsy examination and abnormalities were recorded.
- Statistics:
- Not required.
Results and discussion
- Preliminary study:
- Not performed
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 17 900 - < 69 100 mg/m³ air
- Based on:
- test mat.
- Exp. duration:
- 1 h
- Mortality:
- All animals died at 69100 mg/m3. No mortalities were observed at 17900 mg/m3 or for the control group.
- Clinical signs:
- other: In the control group, animals appeared normal through the exposure and post dose observation periods. In the low exposure concentration group (17.9 mg/L), shallow breathing, gasping, lacrimation, nasal discharge, oral discharge, red and irritated eyes we
- Body weight:
- Body weights were recorded, but body weight gain was not discussed in the report.
- Gross pathology:
- In the control group, the lungs of one female contained multiple (pinpoint to 2 mm) red foci, considered to be an incidental finding. There were no other gross lesions in the remaining nine animals. In the low dose group (17.9 mg/L), a focal (1-2mm) pale, opaque, firm, raised papule was observed on central corneas of each left eye of three males and one female and bilaterally in two females. The central corneas of another female contained a 4 mm, red and white, firm raised papule. Both kidneys of the fifth female contained moderate pelvic dilation which was considered to be incidental. In the high dose group (69 mg/L), there was darkening of the liver in two males and five females. Corneal opacity of the right eye of one male was observed. Mild hydrometra was observed in the uterus of one female, which was considered to be incidental. Two rats exhibited no significant gross lesions.
Any other information on results incl. tables
No futher information available.
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- The acute inhalation LC50 was between 17.9 and 69.1 mg/L for a 1-hour exposure. Using Haber's law (LC50 * 1h/4h), this equates to a 4-hour LC50 of between 4.5-17.3 mg/L. However there was no analytical verification of the achieved concentration and no measurement of particle size, therefore the actual exposure concentrations are unknown.
- Executive summary:
In an acute inhalation toxicity study conducted with the substance TMEDA (Bulab 600), a group of Sprague Dawley rats (5/sex) was exposed to atmospheres containing nominal concentrations of 17900 or 69100 mg/m3 for 1 hour following equilibration. A control group was also tested. All animals exposed to 69100 mg/m3 died; however no mortalities were observed at 17900 mg/m3 or for the control group. In the low exposure group, shallow breathing, gasping, lacrimation, nasal discharge, oral discharge, red and irritated eyes were observed during the exposure period. During the 14-day observation period, the animals showed laboured breathing, nasal discharge, vasodilation and corneal changes. At necropsy, raised papules were observed on the corneas of seven animals. In the high exposure group (69 mg/L), laboured breathing, nasal discharge, convulsions and irritated eyes were observed prior to death. At necropsy, darkening of the liver was reported for two males and five females. Corneal opacity was observed in one animal. Two rats exhibited no significant gross lesions. The acute inhalation LC50 was between 17.9 and 69.1 mg/L for a 1-hour exposure. Using Haber's law, this equates to a 4-hour LC50 of between 4.5-17.3 mg/L (LC50 * 1h/4h). However there was no analytical verification of the achieved concentration and no measurement of particle size, therefore the actual exposure concentrations are unknown.
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