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EC number: 219-976-6 | CAS number: 2589-57-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30.05.-30.06.16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- yes
- Remarks:
- temperature range was exceeded and undercut during testing period. As normal growth was observed, deviation is considered uncritical.
- GLP compliance:
- yes (incl. QA statement)
- Vehicle:
- no
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: 86.81
- Source (laboratory, culture collection): MBM Sciencebridge GmbH (Inst. für Pflanzenphysiologie, Universität Göttingen)
- Age of inoculum (at test initiation):
- Method of cultivation:
ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not):
- Any deformed or abnormal cells observed: - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 20.4-24 °C
- pH:
- start: 7.7 ± 0.1
end: 8.5 ± 0.1 (except highest concentration: pH 7.6) - Nominal and measured concentrations:
- nominal: 1; 3.2; 10; 32; 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Type (delete if not applicable): open (covered with perforated plastic foil)
- Material, size, headspace, fill volume: glass, 20 mL, 45 mL
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter): -
- Renewal rate of test solution (frequency/flow rate):-
- Initial cells density: 4.6E3 cells/mL
- Control end cells density: 5.9E5
- No. of organisms per vessel:-
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates):-
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:-
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Total organic carbon:
- Particulate matter:
- Metals:
- Pesticides:
- Chlorine:
- Alkalinity:
- Ca/mg ratio:
- Conductivity:
- Culture medium different from test medium:
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Sterile test conditions: yes/no
- Adjustment of pH:
- Photoperiod: 16/8
- Light intensity and quality: 5300 Lux
- Salinity (for marine algae):
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: for different reasons three tests in total were performed
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.69 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
- Unusual cell shape:-
- Colour differences: -
- Flocculation: -
- Adherence to test vessels: -
- Aggregation of algal cells: -
- Other: -
- Any stimulation of growth found in any treatment: -
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:-
- Effect concentrations exceeding solubility of substance in test medium:- - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 0.91 mg/L (0.8-1.0 mg/L)
- Other: - Reported statistics and error estimates:
- ToxRat Pro 3.21 was usd for calculations.
- Validity criteria fulfilled:
- yes
- Executive summary:
Three valid experiments were performed.
The first experiment was performed using 5 concentrations ranging from 10 to 100 mg/L. Significant inhibition of algal growth in the lowest concentration of 10 mg/L was observed. Therefore, the first experiment was repeated.
The second and third experiments were performed using 5 concentrations ranging from 1 to 100 mg/L
In the second experiment the analytical samples at the end of the test (72 hours) were not measured due to a technical error.Therefore, the second experiment was repeated.
Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
The biological results were based on the results of the third experiment.
Significant inhibition of algal growth in the third experiment was observed at all concentrations with the exceptions of the lowest concentrated treatment.
At the beginning and every 24 hours during the course the study, the content of the test item in the test solution was determined using HPLC-UV-determination. The concentrations determined at the start of the third experiment were in the range of 85 % and 98 % of the nominal concentration. At the end of the third experiment the determined concentrations were in the range of 23 % and 52 % of the nominal concentration. In the lowest concentration of 1 mg/L nominal concentration the determined concentration was below the limit of quantification at the end of the test. Therefore, the determination of the biological results was based on the geometric mean of the measured concentrations.
The 72h-EC50s of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test itemdimethyl-2,2'-azobisisobutyratewere determined:
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
0.7 mg/L
1.9 mg/L
14 mg/L
> 100 mg/L
Yield
0.7 mg/L
1.9 mg/L
0.69 mg/L
16 mg/L
Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower.
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 14 mg/L
Additional information
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