2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Oct 1989 - Feb 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

4. April 1984

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

EC Number:

404-910-2

EC Name:

2-(4-(diethylaminopropylcarbamoyl)phenylazo)-3-oxo-N-(2,3-dihydro-2-oxobenzimidazol-5-yl)butyramide

Cas Number:

164578-14-7

Molecular formula:

C25H31N7O4

IUPAC Name:

N-[3-(diethylamino)propyl]-4-[(1E)-2-{2-oxo-1-[(2-oxo-2,3-dihydro-1H-1,3-benzodiazol-5-yl)carbamoyl]propyl}diazen-1-yl]benzamide

Test material form:

solid: bulk

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations:
Trial I: 10, 18, 32, 56, 100, 180 mg/L
Trial II: 1, 1.8, 3.2, 5.6 mg/L
- Sampling method:
At the start of the exposure (0 hours) and at the end of the exposure (48 hours), samples for analyses of the freshly prepared and old media were taken from all test replicates.

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
For preparation of the 1 - 5.6 mg/L concentrations 0.02 mL Tween 80 were added to the test item and filled up with water. After sonification for 30 min and stirring for 5 min the solution was filled up to 200 mL end volume with water
.The 10 to 180 mg/L test concentrations were directly weighed in the test vessels and moistened with 0.095 mL Tween 80/L. Afterwards 200 mL water was added and the suspensions were homogenised by sonication and ultra-turrax.
Water: dilution water according to ISO/DIS 7346/1

- Controls:
solvent control: 0.095 mL Tween 80/L

- Chemical name of vehicle (organic solvent, emulsifier or dispersant):

Tween 80
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)):
0.095 mL/L

- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
1-18 mg/L samples: clolorless to yellow solution
18 mg/L: slight precipitation
32-180 mg/L samples: precipation at the begin of experiment, at termination yellow solution

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: STRAUS
- Justification for species other than prescribed by test guideline:
Dahpnia magna is the preferred species in accordance with the test guideline and is bred at the test facility.
- Source: Hoechst AG
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test: no feeding during exposure time
- Food type:
for breeding: green algae (scendesmus subspicatus) and a suspension of Tetra min dry food (Tetra Werke, Melle, Germany)
- Culture: in glass vessels (3 L capacity) with water at 20+/-1°C, 12 h illumination

ACCLIMATION
- Acclimation period: 6 h

Study design

Test type:

static

Water media type:

other: water according to ISO/DIS 7346/1

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Test conditions

Hardness:

no data

Test temperature:

test groups: 20.6 - 21.8°C
control: 20.4 - 21.1°C

pH:

test groups: 8.1 - 8.7
control: 8.1 - 8.3

Dissolved oxygen:

test groups: 8.2 - 9.2 mg/L
control: 8.4 - 9.8 mg/L

Salinity:

no data

Conductivity:

no data

Nominal and measured concentrations:

Nominal concentrations:
1, 1.8, 3.2, 6.5, 10, 18, 32, 56, 100 and 180 mg/L
Measured concentrations:
0.9, 1.6, 2.6, 5.2, 9.4, 19.9, 33.7, 58, 101.5 and 182.5 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL cristallisation dishes (55 mm heigth, 95 mm diameter)
- Material, size, headspace, fill volume: 32 mm
- Aeration: none
- No. of organisms per vessel: 10


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobilisation

VEHICLE CONTROL PERFORMED: yes

RANGE-FINDING STUDY PERFORMED: yes
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

41.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

7.72 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 5

Key result

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

At test item concentration above 10 mg/L the solutions were colored yellow. The measured water quality parameters (i.e. pH, oxygen concentration and temperature) as well as the analysed test item concentrations were within acceptable ranges.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal (and measured) concentrations of the test item, the 48-hour EC50 for Daphnia magna was 7.72 mg/L.

Executive summary:

In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item were determined at the test facility according to OECD 202 (1994).

The study was conducted under static conditions over a period of 48 hours with a test item solution with the nominal concentrations of 1, 1.8, 3.2, 5.6, 10, 18, 32, 56, 100 and 180 mg/L of the test item.

At test item concentration above 10 mg/L the solutions were colored yellow. Ten daphnids were exposed each concentration level and the control.

The concentrations of the test item were analytically verified via photometry in fresh media at the start (0 hours) and in old media at the end of the exposure (48 hours) in each concentration and in the control.

The measured concentrations of the test item were in the range of 89 to 111% of the nominal value.

The measured test item concentrations remained stable within ± 20% of the nominal limit concentration of the test item over the test period.

The validity criteria of the test guideline were fulfilled.