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Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 19 April 2021 to 21 April 2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

13 April 2004

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Batch/Lot number: 381890
Purity: 99.8%

Analytical monitoring:

yes

Details on sampling:

samples were taken and analyzed at the beginning and at the end of the experiment.

Vehicle:

yes

Remarks:

Reconstituted water

Details on test solutions:

A stock solution with a nominal concentration of 100.0 mg/L (nominal) was prepared with direct addition of the test item and mixed into the test medium (ISO Medium).
The test solution was prepared just before introduction of the Daphnia (start of the experiment).

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: (Straus)
- Justification for species other than prescribed by test guideline: Daphnia magna is the standard species of the acute immobilisation test.
- Age at study initiation (mean and range, SD): Less than 24 h old at the beginning of the test.
- Source: Hungarian University of Agriculture and Life Sciences, Department of Aquaculture 2100 Gödöllő, Páter Károly u. 1. – Hungary
- Feeding during test: not fed during the test

ACCLIMATION
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: The Daphnia were fed with concentrated algal suspension of Raphidocelis subcapitata during the holding.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

250 mg/L (as CaCO3)

pH:

in the range of 7.85–7.95

Dissolved oxygen:

in the range of 8.2 – 8.7 mg/L

Nominal and measured concentrations:

100.0 mg/L (nominal);
Measured concentration: 98.8 mg/L at the start and 97.1 mg/L at the end

Details on test conditions:

TEST SYSTEM
- Test vessel: not specified
- Type (delete if not applicable): open
- Volume of solution: at least 5 mL test solution/animal
- Aeration: yes
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Observation of environmental parameters:
The water temperature was determined daily in one control test vessel and continuously with a min/
max thermometer in the climate chamber.
The oxygen concentration and pH were measured in both the control and the test concentration level
(in one test vessel) at the start and at the end of the experiment.
Observation of animals:
The number of mobile and immobilized test animals was observed and recorded 24 and 48 hours af
ter the start of the test. Those animals not able to swim within 15 seconds after gentle agitation of the
test beaker were considered to be immobile.
RANGE-FINDING STUDY
- Test concentrations: 0.01, 0.1, 1, 10, 100.0 mg/L
- Results used to determine the conditions for the definitive study: no significant inhibition was obs
erved at any concentration during the preliminary range-finding test

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The number of immobilised animals and the percentage of immobility were determined at the 24th
and 48th hour (1 animal at 48 hours, 5%).
In addition to immobility, no abnormal behaviour or appearance of test animals was detected.

Results with reference substance (positive control):

The 24h EC50: 0.73 mg/L, (95 % confidence limits: 0.69 – 0.78 mg/L)
This result falls within the acceptable range (0.6 mg/L to 2.1 mg/L) hence this reference study shows
that the acute immobilization test on Daphnia magna is valid in this laboratory.

Validity criteria fulfilled:

yes

Conclusions:

The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.

Executive summary:

Acute toxicity of the test item on Daphnia magna was assessed in an acute immobilisation test, over an
exposure period of 48 hours in a static test system, according to OECD Guideline 202 under GLP.
The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.

Description of key information

Acute toxicity of the test item on Daphnia magna was assessed in an acute immobilisation test, over an exposure period of 48 hours in a static test system, according to OECD Guideline 202 under GLP.
The 48h-EC50 of the test item to daphnia magna is determined as > 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L