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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 300-326-6 | CAS number: 93925-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Between 15 March 1974 and 18 April 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Valid and conclusive pre-GLP study comparable to guideline, basic data reported
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- 2002
- Deviations:
- yes
- Remarks:
- 1 h grading omitted
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: U.S. 16 CFR 1500.42 Federal Hazardous Substances Act (Federal Register, Vol . 38, No. 187, P. 27019, 27 September 1973)
- GLP compliance:
- not specified
- Remarks:
- The experiment was performed before the first GLP standard was defined by FDA in 1976, therefore no formal GLP was possible. Nonetheless it can be assumed that comparable standards applied as the test was conducted in a specialized laboratory.
Test material
- Reference substance name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- EC Number:
- 300-326-6
- EC Name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- Cas Number:
- 93925-25-8
- Molecular formula:
- Complex
- IUPAC Name:
- Phosphonic acid, mixed C12-20-alkyl and C14-18-unsatd. alkyl derivs.
- Test material form:
- liquid: viscous
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: Albino
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Age at study initiation: Young adults
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- TEST MATERIAL
- Amount applied: 0.1 g
- Concentration: Pure test item was used. - Duration of treatment / exposure:
- 3 d (72 h) from test start until test end (no washing)
- Observation period (in vivo):
- 3 d (72 h); the treated eyes were examined at days 1, 2 and 3 following instillation of the test item.
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: No
- Time after start of exposure: The test animals were exposed until test end.
SCORING SYSTEM: Draize method, in accordance with the grading system outlined in the “illustrated Guide for Grading Eye Irritation by Hazardous Substances”.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible within: 24 h (score 0 from this time on)
- Remarks on result:
- other: No 1 h reading performed
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- fully reversible within: 24 h (score 0 from this time on)
- Remarks on result:
- other: No 1 h reading performed
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 3
- Reversibility:
- fully reversible within: 24 h (score 0 from this time on)
- Remarks on result:
- other: No 1 h reading performed; conjunctival redness and chemosis were not differentiated but were grouped as conjunctival scores. The maximum score for chemosis is 4.
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Max. score:
- 4
- Remarks on result:
- not measured/tested
- Irritant / corrosive response data:
- None of the test animals showed any significant difference to the untreated control eye.
- Other effects:
- None
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Not irritating, no difference to control
- Executive summary:
The eye irritation / corrosion properties of the test item was investigated in a pre-GLP study using albino rabbits according to a protocol comparable to the OECD TG 405 (2002) standards. At variance to the guideline the 1 h grading was omitted. As at the 24 h grading no difference to the control eyes was recorded, the study result is not impaired by this deviation. The experiment was performed before the first GLP standard was defined by FDA in 1976 therefore no formal GLP was possible. Nonetheless it can be assumed that comparable standards applied as the test was conducted in a specialized laboratory. The reporting is limited but sufficient information is given. The experiment is deemed valid, conclusive and suitable for assessment with minor restrictions.
A dose of 0.1 g of the test item was instilled in the right eyes of six healthy young adult albino rabbits. The untreated left eyes served as controls. The treated eyes were examined at days 1, 2 and 3 following instillation of the test item and graded in accordance with the Draize method.
No corneal opacity, iritis or conjunctival irritation was observed in any animal at any observation during the test period.
In summary the test item was found not eye irritating with no difference between treatments and control. No indication for classification is given and in accordance with EU CLP Regulation (EC) No. 1272/2008 no classification is required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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