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Diss Factsheets
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EC number: 200-662-2 | CAS number: 67-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Equivalent to guideline study
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Salmonella mutagenicity tests: V. Results from the testing of 311 chemicals
- Author:
- Zeiger E, Anderson B, Haworth S, Lawlor T, Mortelmans K
- Year:
- 1 992
- Bibliographic source:
- Environ Mol Mutagen 19: 2-141
- Reference Type:
- other: database
- Title:
- Acetone, CAS Number: 67-64-1, Study type: Salmonella, Study ID: 230717
- Author:
- National Toxicology Program (NTP)
- Year:
- 1 986
- Bibliographic source:
- http://ntp-apps.niehs.nih.gov/ntp_tox/index.cfm
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Acetone
- EC Number:
- 200-662-2
- EC Name:
- Acetone
- Cas Number:
- 67-64-1
- Molecular formula:
- C3H6O
- IUPAC Name:
- propan-2-one
- Details on test material:
- - Name of test material (as cited in study report): acetone
- Analytical purity: > 99%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 97, TA 98, TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced hamster liver S-9 mix and Aroclor 1254-induced rat liver S-9 mix; S-9 concentrations were 10% and 30% except for strain TA 1537 with 30% only
- Test concentrations with justification for top dose:
- 0, 100, 333, 1000, 3333, 10,000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
Controls
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation in suspension in capped tubes to prevent evaporation
DURATION
- Preincubation period: 20 min
- Exposure duration: 2 days
SELECTION AGENT (mutation assays): histidine inpendency
NUMBER OF REPLICATIONS: triplicate plus repeat experiment
DETERMINATION OF CYTOTOXICITY
- Method: decrease of his+ colonies or clearing in the density of the background lawn or both
POSITIVE CONTROLS
- without metabolic activation: sodium azide for TA 1535 and TA 100, 9-aminoacridine for TA 97 and TA 1537, 4-nitro-o-phenylenediamine for TA98 and TA 1538
- with metabolic activation: 2-aminoanthracene - Evaluation criteria:
- mutagenic or weakly mutagenic response: reproducible, dose-related response over solvent control
questionable response: results of inidvidual trials not reproducible, no dose-related response, only single doses producing increases in revertants
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with
- Genotoxicity:
- ambiguous
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: other: 10% and 30% hamster liver S-9 and 10% rat liver S-9 as activation system
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Overview on test results
Strain | metabolic activation | Overall result | ||||
no | 10% hamster S-9 | 30% hamster S-9 | 10% rat S-9 | 30% rat S-9 | ||
TA 1535 | - | - | - | - | - | - |
TA 1537 | - | no data | - | no data | - | - |
TA 97 | - | - | - | - | questionable a | - |
TA 98 | - | - | - | - | - | - |
TA 100 | - | - | - | - | - | - |
a mean revertants at 0, 100, 333, 1000, 3333, 10,000 µg/plate: 127, 174, 168, 177, 177, 173
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
There was no evidence of a genotoxic potential of acetone in the Salmonella typhimurium reverse mutation assay with and without metabolic activation up to a concentration of 10 mg/plate. - Executive summary:
The genotoxic potential of acetone was investigated in Salmonella typhimurium strains TA 1535, TA 1537, TA 97, TA 98 and TA 100 with and without metabolic activation in concentrations ranging from 100 to 10,000 µg/plate with a test protocol comparable to OECD guideline 471. Test results were negative except for a questionable result (constant increase of revertant rate above vehicle control over the whole concentration range tested) with the strain TA 97 in the assays with metabolic activation with 30 % rat liver S-9. In contrast, all other tests with TA 97 and with metabolic activation with 10 % or 30 % hamster liver S-9 or 10 % rat liver S-9 were unequivocally negative. In summary, there was no evidence of a genotoxic potential of acetone in the Salmonella typhimurium reverse mutation assay with and without metabolic activation up to a concentration of 10 mg/plate.
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