6,10-dimethylundeca-3,5,9-trien-2-one

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study, however a solubilser was used

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: German national standard DIN 38412, part 9

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

6,10-dimethylundeca-3,5,9-trien-2-one

EC Number:

205-457-1

EC Name:

6,10-dimethylundeca-3,5,9-trien-2-one

Cas Number:

141-10-6

Molecular formula:

C13H20O

IUPAC Name:

6,10-dimethylundeca-3,5,9-trien-2-one

Details on test material:

- Name of test material (as cited in study report): 2-Pseudoionon

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution with a nominal concentration of 100 mg/l was prepared. Chremophor was used as a solubiliser.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: SAG 86.81
- Source (laboratory, culture collection): Algae collection Goettingen, Germany

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.6 °C

pH:

7.74 - 8.83

Nominal and measured concentrations:

Nominal test concentrations (mg/l):
0.5,
1.0,
2.0,
5.0,
10.0,
control

Details on test conditions:

TEST SYSTEM
- Initial cells density: 12000 N/ml
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 5


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter, at 300 - 780 nm


TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.5, 5, 50, 500 mg/l

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

1.09 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

2.02 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

96 h

Dose descriptor:

EC10

Effect conc.:

1.66 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

2.62 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 5

Duration:

72 h

Dose descriptor:

EC10

Effect conc.:

0.53 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

1.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 7

Duration:

96 h

Dose descriptor:

EC10

Effect conc.:

0.63 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 8

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

1.26 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass