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Diss Factsheets
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EC number: 200-143-0 | CAS number: 52-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Stock solutions of the test material ware prepared in distilled water. One litre of each test solution was prepared by direct addition of aliquots of stock solution to dilution water.
- The dilution water was aerated for > 2 hours before use. The pH of the dilution water was 8.25 ± 0.25.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Test organism: Daphnia magna STRAUS, obtained from continuous laboratory cultures
- Holding: the stock cultures of Daphnia were maintained in a reconstituted water medium, identical to the test dilution water, at a temperature of 20.0 +/- 1°C and a photoperiod of 16 h light:8 h dark
- Feeding: the cultures were fed a defined diet of algae (Chlorella vulgaris) and yeast
- Daphnia 12 +/- 12 hours old were used for testing - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 19.5 - 20.0°C
- pH:
- 8.20 to 8.40
- Dissolved oxygen:
- 8.4 to 9.4 mg/L
- Nominal and measured concentrations:
- - Concentrations tested (nominal): 0.032, 0.056, 0.1, 0.18, 0.32, 0.56, 1.0, 3.2 and 5.6 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessels: Borosilicate glass beakers
- Test volume: 200 ml of test solution
- Replicates per concentration: 4
- Organisms per replicate: 5
- Organisms per concentration: 20
FURTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark
- Light intensity: ca. 1500 lux
- Feeding: none - Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.56 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- EC50 (24 h) = 2.9 mg/L
- Validity criteria fulfilled:
- yes
Reference
Description of key information
Acutely toxic for aquatic organisms.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 1.4 mg/L
Marine water invertebrates
Marine water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 3.5 mg/L
Additional information
The acute toxicity of Bronopol was examined in a static test system according to methods comparable to OECD guideline 202 with Daphnia magna as test organism [The Boots Company 1981]. The 48 h EC50 value was determined to be 1.4 mg/L (nominal).
A supporting study is available which was conducted according to ISO guideline 14669 with the saltwater species Acartia tonsa. At test termination after 48 h an EC50 of 3.5 mg/L (nominal) was determined [Knoll MicroCheck 1998].
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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