Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 289-142-4 | CAS number: 86024-59-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From August 5th to December 25th, 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test conducted according to internationally accepted testing guidelines.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Trisodium hydrogen [2-[[α-[[3-[[4-chloro-6-[ethyl[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulphophenyl]azo]benzyl]azo]-4-sulphobenzoato(6-)]cuprate(4-)
- EC Number:
- 289-142-4
- EC Name:
- Trisodium hydrogen [2-[[α-[[3-[[4-chloro-6-[ethyl[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulphophenyl]azo]benzyl]azo]-4-sulphobenzoato(6-)]cuprate(4-)
- Cas Number:
- 86024-59-1
- Molecular formula:
- C33H24ClCuN9O15S4.3Na
- IUPAC Name:
- Trisodium hydrogen [2-[[α-[[3-[[4-chloro-6-[ethyl[4-[[2(sulphooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-hydroxy-5-sulphophenyl]azo]benzyl]azo] -4-sulphobenzoato(6-)]cuprate(4-)
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Japan, Inc. (Hino Breeding Center, Shiga, Japan).
- Age at study initiation: six-week old.
- Weight at study initiation: 221 -239 g for males; 164 - 183 g for females.
- Fasting period before study: fasting period about 20 hours before the dosing and 4 hours after the dosing.
- Housing: two or three animals of the same sex were placed in a suspended aluminium cage with wire-mesh floor (floor 22.4 cm x 41.9 cm, height 20.0 cm; YAMATO Scientific Co., Ltd., Tokyo, Japan). The cage and tray were changed at least once and twice weekly, respectively.
- Diet: rats were fed laboratory animal chows (CRF-1 sterilized by 60Co at 30 kGy, pellet type, Oriental Yeast Co., Ltd., Tokyo, Japan), ad libitum.
- Water: tap water passed through a filter apparatus, ad libitum except for the 4 hours after dosing.
- Acclimation period: quarantine and acclimatization period of 11 days.
ENVIRONMENTAL CONDITIONS
- Temperature:24 ± 2 °C
- Humidity: 55 ± 15 %
- Air changes: more than 10 times per hour.
- Photoperiod: 12 hours light/dark cycle by fluorescent lighting system.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 200 mg/ml
- Dosing volume: 10.0 ml/kg bw - Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days.
- Frequency of observations and weighing: observation of clinical signs and mortality was carried out at 10 and 30 minutes, 1, 2 and 4 hours after administration and once daily for 2 weeks thereafter. Each animal was weighed on Days 0, 7 and 14 during the observation period.
- Necropsy of survivors performed: yes. At the end of observation period, all animals were exsanguinated from the abdominal aorta under pentobarbital anaesthesia, and were necropsied for gross pathological examination. - Statistics:
- Analysis of variance in one way classifications was performed for mean body weight and mean body weight gain. With regard to the parameters in which a significant difference was found at the 5 % level, the least significant difference (LSD) method was used as a test for significant difference compared with the control group.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No dead animals were found in any groups.
- Clinical signs:
- other: Fecal excretion of blue substance was observed in males and females at 2000 mg/kg. This clinical sign appeared 4 hours after dosing and disappeared within 3 days.
- Gross pathology:
- Retention of white substance in the urinary bladder was found in both control and treated groups. There was no treatment-related finding in the gross pathological examination.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information according to the CLP Regulation (EC 1272/2008) Criteria used for interpretation of results: EU
- Conclusions:
- LD50 (rat) (males/females) > 2000 mg/kg bw
- Executive summary:
Acute oral toxicity of the test substance was examined in rats at the dosage of 2000 mg/kg in both sexes. The test material was suspended in distilled water at the concentration of 200 mg/ml, and orally administrated at 10.0 ml/kg body weight. None of the animals employed in the experiment died. Fecal excretion of blue substance was observed in both sexes at 2000 mg/kg. The mean body weight gain on day 0 to day 7 at 2000 mg/kg males V was lower than that of control. No treatment-related findings were observed by gross pathological examination.
Conclusion
LD50 (rat) (males/females) > 2000 mg/kg bw
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)