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EC number: 205-778-7 | CAS number: 150-90-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental result performed using standard methods of analysis
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the toxic effects of the test compound on the Zebra fish(Danio rerio) for the test material
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Source:Anytime Pet, Jariphataka, Nagpur
- Length at study initiation : average 1.8 cm
- Weight at study initiation: 0.082g (average)
- Method of breeding: The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions alongwith proper feed and aeration.
ACCLIMATION
- Acclimation period: 7 days
- Type and amount of food during acclimation:
- Feeding frequency during acclimation: Daily once
-Photoperiod: 16 hours light and 8 hours dark - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- observations were made at 24, 48, 72 and 96 hours
- Hardness:
- 155.5 mg of CaCO3
- Test temperature:
- 23 °C
- pH:
- 7.1
- Dissolved oxygen:
- 7.0 mg/l
- Nominal and measured concentrations:
- 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Bowl aquari
- fill space : 2 liter
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Adjustment of pH: 7.4
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- mortality was not observed
- Sublethal observations / clinical signs:
8.1. Mortality and symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
Mortality (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
-
-
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
Nominal
Concentration
(mg/l)
Number of
fish
symptom (cumulated) after
3h
6h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A: apathy
N: Narcotic-like state
C: Swimming near water surface
M: Slow movements compared to control
NS: No abnormal symptoms
S: Surfacing behaviour
L: Loss of equilibrium
SM: Shimming movement
H1: Hyperactivity
H2: Hypo activity
B: Bending behavior
F: Fin rot
IS: Invert Swimming
SB: Swimming behaviour
RF: Respiratory Function
P: Pigmentation
pH and oxygen content:
Nominal
Concentration
(mg/l)
Number of
fish
pH readings after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.3
7.3
7.3
7.5
7.5
7.5
7.6
7.6
100
4
4
7.4
7.4
7.5
7.5
7.7
7.7
7.8
7.8
Nominal
Concentration
(mg/l)
Number of
fish
Oxygen content (mg/L) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
7.0
7.0
6.9
6.9
6.7
6.7
6.6
6.6
100
4
4
6.9
6.9
6.8
6.8
6.7
6.7
6.6
6.6
Nominal
Concentration
(mg/l)
Number of
fish
Temperature (°C) after
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
Control
4
4
23.2
23.2
23.4
23.4
23.9
23.9
23.9
23.9
100
4
4
23.2
23.2
23.4
23.4
23.9
23.9
23.9
23.9
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for the test material on Zebra fish(Danio rerio)was found to be > 100 mg/L.
- Executive summary:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.
Reference
Description of key information
Short term toxicity to fish:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Short term toxicity to fish:
Toxicity of test material was evaluated on fish based on study report of the test material and data from authoritative database.Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 100 mg/L,respectively.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.
In an authoritative data base , the short term toxicity test on fish was evaluated according to OECD guideline 203 . The LC50 value of the test substance to fish is found to be >100 mg/L. This value indicates that the substance is not likely to exhibit short term toxicity to fish.Based on the LC50, it can be consider that the chemical was toxic and can be consider to be not classified as per the CLP classification criteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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