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EC number: 947-727-8 | CAS number: 568591-00-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 - 06 Dec 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 Apr 2004
- Qualifier:
- according to guideline
- Guideline:
- other: "Daphnia sp., Acute Immobilization Test" in "Testing Methods for New Chemical Substances", Japan
- Version / remarks:
- March 31, 2011, No. 0331-7, Pharmaceutical and Food Safety Bureau, Ministry of Health, Labour and Welfare; March 29, 2011, No. 5, Manufacturing Industries Bureau, Ministry of Economy, Trade and Industry; No. 110331009, Environmental Policy Bureau, Ministry of the Environment, Japan; latest revision, December 21, 2015, No. 1221-1, Pharmaceutical and Food Safety Bureau, Ministry ofHealth, Labour and Welfare; December 9, 2015, No. 1, Manufacturing Industries Bureau, Ministry of Economy, Trade and Industry; No. 1512211, Environmental Policy Bureau, Ministry of the Environment, Japan
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reaction mass of 2-ethyl-4-methyl-1H-imidazole-1-propiononitrile and 2-ethyl-5-methyl-1H-imidazole-1-propiononitrile
- EC Number:
- 947-727-8
- Cas Number:
- 568591-00-4
- Molecular formula:
- C9H13N3
- IUPAC Name:
- Reaction mass of 2-ethyl-4-methyl-1H-imidazole-1-propiononitrile and 2-ethyl-5-methyl-1H-imidazole-1-propiononitrile
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- - Concentrations: All test concentrations and the control at the start (0 h) and end (48 h) of exposure.
- Sampling method: At the start of exposure, samples were taken from a separate replicate. At the end of exposure, samples were taken from the middle layer of the test solution of every test vessel. The sample volume was 10 mL.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution with a nominal concentration of 100 mg/L was prepared by mixing and stirring 0.2 g test item into 2000 mL dilution water. Required volumes of the stock solution were diluted with dilution water to prepare the test solutions. For the 100 mg/L level, the stock solution was divided into each test vessel for test solution.
- Differential loading: No
- Controls: Dilution water without test item
- Evidence of undissolved material: The test solutions in all exposure levels and the control were colorless and clear at the start and end of exposure.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain/clone: Clone A
- Source: University of Sheffield, UK (09 Jul 1990)
- Age of daphnids at test start: < 24 h old daphnids. The parent animals used for the test were from the same lot, were 24 d old and had a survival rate of 100%.
- Breeding conditions: Water: dechlorinated tap water; Water temperature: 20 ± 1 °C; Photoperiod: 16 h light/8 h dark; Feeding: 0.1 - 0.2 mg C (organic carbon content) Chlorella vulgaris per day per daphnid.
- Feeding during test: No feeding during test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 32 mg/L (Ca and Mg, dilution water)
- Test temperature:
- 19.8 - 19.9 °C
- pH:
- 7.7 - 8.6
- Dissolved oxygen:
- 8.8 - 9.0 mg/L
- Nominal and measured concentrations:
- Control, 9.53, 17.1, 30.9, 55.6, and 100 mg/L (nominal)
< 0.100, 9.91, 17.7, 31.9, 57.4, and 106 mg/L (geometric mean measured 0 - 72 h) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beakers filled with 100 mL test solution and covered with a transparent plastic lid.
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Total organic carbon: < 0.5 mg/L
- Metals: > LOD
- Pesticides: < LOD
- Chlorine: < 0.02 mg/L
- Alkalinity: 36 mg/L
- Ca/mg ratio: 9.1 mg/L Ca, 2.2 mg/L Mg
- Conductivity: 13 mS/m
- Culture medium different from test medium: Culture medium same as test medium
- Intervals of water quality measurement: Dissolved oxygen, pH and temperature were measured at the start and end of exposure.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light/ 8 h dark
- Light intensity: Room light
EFFECT PARAMETERS MEASURED:
- Immobilization and symptoms: after 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: 1.0, 10.0, and 100 mg/L (nominal)
- Results used to determine the conditions for the definitive study: Yes, 70% immobility observed after 48 h in the 100 mg/L treatment level. Observed symptoms: immobilization and reduced activity. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (tested periodically)
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 99.9 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% confidence limits
- Remarks:
- 84.6 - 143 mg/L
- Details on results:
- - Behavioural abnormalities: None in the control. After 48 h, reduced activity was observed at 30.9 mg/L (2 individuals), 55.6 mg/L (16 individuals), and 100 mg/L (6 individuals). Immobilization was observed at 55.6 mg/L (1 individual) and 100 mg/L (10 individuals).
- Mortality of control: 0%
- Other adverse effects control: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test solutions in all exposure levels and the control were colorless and clear at the start and end of exposure.
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes, the effect value was within the stipulated range (mean ± 2SD: 0.1 - 0.35 mg/L, n = 102) to background data in the testing facility.
- Relevant effect levels: EC50 (48 h) = 0.12 mg/L potassium dichromate
- Period of reference test: 18 - 20 Oct 2017 - Reported statistics and error estimates:
- Probit analysis
Any other information on results incl. tables
VALIDITY OF STUDY
The study fulfilled the validity criteria of the guideline (Table 1).
Table 1: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% immobilization in the control. |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
Dissolved oxygen concentrations in the control and all treatment levels were 9.0 mg/L at the end of exposure.
|
Yes |
ANALYTICAL RESULTS
At the start of exposure, the measured test item concentrations were 9.77 – 103 mg/L (101 – 103% of the nominal concentrations). At the end of exposure, the measured test item concentrations were 10.0 – 108 mg/L (105 – 108% of the nominal concentrations). Hence, the measured test item concentrations were within ± 20% of the nominal concentrations (Table 2).
Table 2. Measured test item concentrations in the test solutions.
nominal concentration [mg/L] |
measured concentration [mg/L] (percentage of measured concentration versus the nominal concentration) |
||
At test start |
At test end |
Geometric mean |
|
Control |
n.d. |
n.d. |
-- |
9.53 |
9.77 (103) |
10.0 (105) |
9.91 (104) |
17.1 |
17.5 (102) |
18.0 (105) |
17.7 (104) |
30.9 |
31.4 (101) |
32.4 (105) |
31.9 (103) |
55.6 |
56.5 (102) |
58.3 (105) |
57.4 (103) |
100 |
103 (103) |
108 (108) |
106 (106) |
n.d.: < 0.10 mg/L
BIOLOGICAL RESULTS
After 48 h, the minimum concentration causing 100% immobility was > 100 mg/L because it was not obtained from the concentration range of the definitive study. The maximum concentration causing no immobility after 48 h was 30.9 mg/L (Table 3).
Table 3. Immobilization.
nominal concentration [mg/L] |
vessel |
24 h |
48 h |
||
number of immobilized daphnids/total daphnids |
immobility [%] |
number of immobilized daphnids/total daphnids |
immobility [%] |
||
Control |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
9.53 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
17.1 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
30.9 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
55.6 |
A |
1/5 |
5 |
1/5 |
5 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
100 |
A |
1/5 |
35 |
1/5 |
50 |
B |
1/5 |
3/5 |
|||
C |
2/5 |
3/5 |
|||
D |
3/5 |
3/5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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