Registration Dossier
Registration Dossier
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EC number: 200-309-2 | CAS number: 57-06-7
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Mutagenicity of Isothiocyanates, Isocyanate and Thioureas on Salmonella Typhimurium
- Author:
- Tsutomu Yamaguchi
- Year:
- 1 980
- Bibliographic source:
- Agric. Biol. Chem.,44 (12), 3017-3018, 1980
Materials and methods
- Principles of method if other than guideline:
- The mutagenicity of isothiocyanates and related compounds was tested by the method of Ames et al. with some modifications.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Allyl isothiocyanate
- EC Number:
- 200-309-2
- EC Name:
- Allyl isothiocyanate
- Cas Number:
- 57-06-7
- Molecular formula:
- C4H5NS
- IUPAC Name:
- 3-isothiocyanatoprop-1-ene
- Test material form:
- liquid
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 100
- Species / strain / cell type:
- S. typhimurium, other: TA1535, TA1537,TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 Mix
- Vehicle / solvent:
- DMSO
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: TA100, TA98
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Species / strain:
- S. typhimurium, other: TA1535, TA1537, TA1538
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- There was no activating effect on mutagenicity by the pretreatment of these samples with S-9 Mix.
Applicant's summary and conclusion
- Conclusions:
- It was found that all of the isohiocyanates tested showed mutagenicity on Salmonella typhimurium TA100, though the degree varied with each sample. Especially, allyl isothiocyanate have the highest potency among these compunds.
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