disodium 4,4'-bis[(prop-2-enoyl)amino][1,1'-biphenyl]-2,2'-disulfonate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

27 Feb - 01 Mar 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

2004

GLP compliance:

yes (incl. QA statement)

Remarks:

Landesamt für Umwelt, Rheinland-Pfalz, Mainz, Germany

Test material

Test material information

Constituent 1

Reference substance name:

disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate

EC Number:

812-549-0

Cas Number:

881539-89-5

Molecular formula:

C18H14N2Na2O8S2

IUPAC Name:

disodium 4-[(1-hydroxyprop-2-en-1-yl)amino]-4'-(prop-2-enamido)-[1,1'-biphenyl]-2,2'-disulfonate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: Blank control and 100 mg/L (at 0 and 48 h)

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A solution containing 102.4 mg/L in dilution water was prepared.
- Controls: dilution water only

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Age at study initiation (mean and range, SD): 0 - 18 h
- Method of breeding: similar to the method described in the OECD guideline: vessels: preserving glasses (nominal volume 2 L), medium: M4-Medium (recipe of ELENDT), food: green algae (Desmodesmus subspicatus), medium renewal: twice a week, photo period: 16/8 hours (using neon tubes), temperature 20 ± 2 °C
- Source: Umweltbundesamt Berlin (27. September 2007)

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS:
19 hours before the start of the test, the adult animals were separated from the young. 1 hour before test start, adults were caught with the help of a glass tube, and the newborn daphnia, aged between 0 and 18 hours, were sieved from the medium and immediately placed into a beaker containing dilution water. After the settling-in period, animals which showed no apparent damage were used for the test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.50 mmol/L, 250 CaCO3/L

Test temperature:

19.8 - 21.3°C

pH:

7.7 - 7.8

Dissolved oxygen:

8.6 - 9.0 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0 (control), 100 mg/L
Measured concentrations initial (t-=0) /test end (t-72h): < LOQ, 106.03/111.90 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers, nominal volume 50 mL, tall shape
- Volume of solution: 20 ± 5 mL
- Aeration: no
- Renewal rate of test solution: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO test water according to OECD 202
- Culture medium different from test medium: yes, switching from M4-medium (husbandry) to ISO test water has been shown not to cause any detrimental effects for test daphnia.
- Intervals of water quality measurement: pH and dissolved oxygen (0, 48 h)

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilised daphnia (24, 48 h)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: only one of the daphnids was immobilised which can be stated as not significant.

Results with reference substance (positive control):

24h-EC50: 1.9 mg/L (within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L as specified in the OECD guideline)

Any other information on results incl. tables

Table 1: Calculated Test item Concentrations

 

Nominal Concentration Test Item	Measured Concentration Test Item t = 0 h	Measured Concentration Test Item t = 48 h	% of Nominal concentration t = 0 h	% of Nominal concentration t = 48 h
mg/L	mg/L	mg/L	%	%
Blank control	< LOQ	< LOQ	--	--
100	106.0	111.9	106	112

LOQ (Limit of quantification) = 4 mg/L

Table 2: Immobility

 

Nominal Concentration in mg/L	Immobility 24 hours					Immobility 48 hours
	absolute				in %	absolute				in %
Blank control	0	0	0	0	0	0	1	0	0	5
100	0	0	0	0	0	0	0	0	0	0

Table 3: Validity criteria for OECD 202.

Criterion from the guideline	Outcome	Validity criterion fulfilled
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized.	5%	yes
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels.	≥ 8.6 mg/L	yes

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

For further details please refer to “Any other information on results incl. tables”.