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Diss Factsheets
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EC number: 202-854-1 | CAS number: 100-46-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The method described in the publication was conducted in compliance with an OECD guideline.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Biodegradation and Bioaccumulation Data of Existing Chemicals based on the CSCL Japan
- Author:
- Anonymous
- Year:
- 1 992
- Bibliographic source:
- Japan Chemical Industry Ecology-Toxicology & Information Center
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Benzylamine
- EC Number:
- 202-854-1
- EC Name:
- Benzylamine
- Cas Number:
- 100-46-9
- Molecular formula:
- C7H9N
- IUPAC Name:
- 1-phenylmethanamine
- Details on test material:
- - Name of test material (as cited in study report): Benzylamine
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: sludge samplings from different sewage plants, rivers, bays and a lake
- Details on inoculum:
- - Source of inoculum/activated sludge:
Sludge sampling was made at the following 10 places in Japan: Fukogawa city sewage plant, Fukashiba industry sewage plant, Nakahama city sewage plant, Ochiai city sewage plant, Kitakami river, Shinano river, Yoshino river, Lake Biwa, Hiroshima bay, Dookai bay.
Sampling method: (1) City sewage: returned sludge of sewage plants were collected; (2) Rivers, lakes and sea: Surface water and surface soil which is in contact with atmosphere were collected.
- Culture:
5 L of the filtrate of the supernatant of an activated sludge in the present use was mixed with 500 mL of the filtrate of the supernatant of a newly collected sludge respectively and the mixture was cultured at pH 7 under the sufficient aeration (using prefiltered open air). About 30 minutes after ceasing the aeration to the sludge mixture, supernatant corresponding to about 1/3 of the whole volume was removed. Then the equal volume of dechlorination water was added to the remaining portion and aerated again, followed by addition of synthetic sewage, concentration of that portion was to be 0.1% (w/v). This procedure was repeated once every day. The culturing was carried out at 25 +/- 2°C. (Synthetic sewage: Glucose, peptone and monopotassium phosphate were dissolved in dechlorination water. Each conentration of them was to be 5% (w/v) and the solution was adjusted to pH 7 with sodium hydroxide.)
- Preparation of inoculum for exposure:
(1) Measurement of concentration of suspended solid: Method in accordance with japanses Industrial standards (JIS) K 0102-1986-14.1.
(2) Preparation of basal culture medium: Each 3 mL of solutions A, B, C and D which are described in JIS K 0102-1986-21, were made up to 1000 mL with purified water and then pH of this solution was adjusted to 7.
(3) Reference substance: Aniline was used as reference substance.
- Preparation of test solutions:
water + test substance (300 mL purified water + 30 mg of test substance), sludge + test substance (300 mL basal culture medium + 30 mg or of test substance), sludge + anailine (300 mL basal culture medium + 30 mg anailine), control blank (300 mL basal culture medium).
Activated sludge was added to each test vessel containing basal culture medium, in such a way that concentration of suspended soil reached to 30 mg/L.
- Instrument and condition of cultivation:
closed system oxygen measuring apparatus; vessel: 300 mL of volume; absorbent for carbon dioxide: soda lime No.1 (extra pure reagent);
test solution was stirred by magnetic stirrer;
cultivating temperature: 25 +/- 2°C
cultivating duration: 14 days - Duration of test (contact time):
- 14 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Remarks:
- and determination of total organic carbon
- Details on study design:
- TEST CONDITIONS
- Test temperature: 25 +/- 2°C
- pH: 7
- pH adjusted: yes
TEST SYSTEM
- Culturing apparatus: Closed system oxygen consumption measuring apparatus (Coulometer: Ohkura Electric Co., Ltd.)
CONTROL AND BLANK SYSTEM
- Inoculum blank: Vessel containing 300 mL of basal culture medium into which no test substance or control substance was added.
- Toxicity control: Aniline was used as a reference substance.
Reference substance
- Reference substance:
- aniline
Results and discussion
- Preliminary study:
- No data
- Test performance:
- After termination of cultivation, total organic carbon and test substance in test solutions were determined. The pH of the test solutions (water + test substance and sludge + test substance) were measured. The mean degree of biodegradation as calculated from BOD was in the range of 52-75% and 62-91% ( calculated from theoretical oxygen demand).
% Degradationopen allclose all
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 52 - 75
- Sampling time:
- 14 d
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 62 - 91
- Sampling time:
- 14 d
- Remarks on result:
- other: calculated from theoretical oxygen demand
- Details on results:
- The mean degree of biodegradation as calculated from BOD was in the range of 52-75% and 62-91% ( calculated from theoretical oxygen demand).
BOD5 / COD results
- Results with reference substance:
- As not stated otherwise, the percentage of biodegradation of anailine calculated by BOD value were beyond 40% and 60% at day 7 and 14 after initiation of the test, respectively.
Any other information on results incl. tables
No other information.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The mean degree of biodegradation as calculated from BOD was in the range of 52-75% and 62-91% (calculated from theoretical oxygen demand) and, thus, benzylamine is considered readily biodegradable.
- Executive summary:
The study was conducted according to OECD 301C, Ready Biodegradabilty: Modified MITI Test (I).
Sludge sampling was made at 10 different places in Japan from sewage plants, rivers, bays and a lake. Activated sludge (30 mg/L) was incubated with benzylamine (final concentration of 100 mg/L) for 14 days at 25°C in a closed system oxygen measuring apparatus.
After termination of cultivation, total organic carbon and test substance in test solutions were determined. The pH of the test solutions (water + test substance and sludge + test substance) were measured.
The mean degree of biodegradation as calculated from BOD was in the range of 52-75% and 62-91% (calculated from theoretical oxygen demand) and, thus, benzylamine is considered readily biodegradable.
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