Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 453-480-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 April 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Since HATCOL 5127 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/1 was tested in duplicate.
Reference substance
A solution of 3,5-dichloraphenol was prepared by dissolving 125.8 mg in 2.5 ml 1 N NaOH, diluting it to approximately 7.5 ml with Milli-Q water and adding under stirring 1 N H2S04 to the paint of incipient precipitation. Finally the solution was diluted to 250 m1 with Milli-Q water. The pH was 8.0. Aliquots 50 ml were stored in a freezer until use. On the day of testing the reference substance solution was defrosted at room temperature.
Three concentrations were tested: 3.2, 10, 32 mg/l. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Test system: Micro-organisms in activated sludge.
Source: Municipal sewage treatment plant: Waterschap de Maaskant', 's-Hertogenbosch, the Netherlands, receiving predominantly domestic sewage.
Number of micro-organisms: Number of micro-organisms was determined as the amount of Mixed Liquor Suspended Solids (MLSS) per litre test medium.
Preparation of the sludge: The sludge was coarsely sieved, washed and diluted with tap-water. A small amount of the sludge was weighed and dried at ca. 105°C to determine the amount of suspended solids (2.6 g/l of sludge, as used for the test). The pH on the day of testing was 7 .9. The batch of sludge was used on the subsequent day, therefore 50 ml of synthetic sewage feed was added to each litre of activated sludge at the end of the day. The sludge was kept aerated at test temperature until use.
Rationale: Recognized by international guidelines as the recommended test system. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Hardness:
- Not specified
- Test temperature:
- 18.6 and 19.2°C
- pH:
- 7.9
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable.
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Norminal 100 mg/L
- Details on test conditions:
- Test procedure and conditions
Contact time: 30 minutes, during which aeration and stirring took place.
Vessels: AII glass, 300 ml oxygen bottles and 1 1 test bottle
Milli-RO / Milli-Q water: Tap-water purified by reverse osmosis (Milli-RO) and subsequently passed over activated carbon and ionexchange caridges (Milll-Q) (Millipore Corp., Bedford, Mass., USA).
Synthetic sewage feed: 16 g peptone
11 g meat ex1ract
3 g urea
0.7g NaCI
0.4 g CaCl2.2H20
0.2 g MgS0,.7H20
2.Bg K,HPO,
Dissolved in Milli-Q water, made up to 1 1 and filtered. The pH was 7.0.
Air supply: Clean, oil-free air.
Oxygen meter: WTW inolab Oxi Level 2 supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
Recorder: Flatbed recorder SE 102 (Kipp & Zonen).
Performance of the test:
The synthetic sewage feed (16 ml) and an adequate amount of the test substance were mixed and made up to 300 ml with Milli-RO water. Activated sludge (200 ml) was added to provide a final volume of 500 ml. The mixture was then aerated in a 11 bottle during the contact time, using a pipette as an aeration device.
Then a well mixed sample of the contents was poured into a 300 ml oxygen bottle, and the flask was sealed with an oxygen electrode connected to a recorder, forcing the air out of the vessel. Oxygen consumption was measured and recorded for at least 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer. The pH and temperature were determined in the remaining part of the reaction mixture. This procedure was repeated for the duplicate concentration. ln each test series two controls without test substance were tested, one at the start and one at the end.
Each batch of activated sludge was checked for sensitivity by testing the reference substance 3,5-dichlorophenol. - Reference substance (positive control):
- yes
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- No inhibition of respiration rate of the sludge was recorded at 100 mg HATCOL 5127 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Experimental conditions: The temperature of the test medium was between 18.6 and 19.2°C. - Results with reference substance (positive control):
- Reference substance was observed to inhibit the total respiration rate.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test, HATCOL 5127 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
This concentration was prepared by direct addition of HATCOL 5127 to the test vessels and exceeded the solubility of HATCOL 5127 in water. - Executive summary:
The influence of HATCOL 5127 on the respiration rate of activated sludge was investigated after a contact time of 30 minutes.
The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L133, adopted May 30, 1988.
HATCOL 5127 was a light yellow liquid.
Since HATCOL 5127 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/l was tested in duplicate.
No inhibition of respiration rate of the sludge was recorded at 100 mg HATCOL 5127 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, under the conditions of this present test, HATCOL 5127 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
Reference
Controls (C) and HATCOL 5127 (T): pH, oxygen concentration, oxygen consumption and percentage inhibition
Flask |
Concentration (mg/l) |
Oxygen conc. at the start (mg o2/l) |
Oxygen consumption mg/o2/l/hr |
% inhibition |
pH |
C1 |
0 |
8.3 |
29 |
- |
8.0 |
C2 |
0 |
8.5 |
31 |
- |
7.9 |
Mean C1+C2 |
|
|
30 (7%) |
|
|
T1 |
100 |
6.9 |
32 |
-7 |
7.9 |
T2 |
100 |
7.1 |
31 |
-3 |
7.8 |
Negative values indicate stimulation of respiration rate of the sludge. These values are considered as not significant.
Reference substance (R; 3, 5-dichlorophenol): pH, oxygen concentration, oxygen consumption and percentage inhibition
Flask |
Concentration (mg/l) |
Oxygen conc. at the start (mg o2/l) |
Oxygen consumption mg/o2/l/hr |
% inhibition |
pH |
R1 |
3.2 |
8.1 |
25 |
17 |
8.0 |
R2 |
10 |
8.2 |
15 |
50 |
7.9 |
R3 |
32 |
8.9 |
8 |
73 |
7.9 |
Determination of the EC50-value for 3,5-dichlorophenol
% inhibition
Concentration (mg/L) |
X Log conc. (mg/L) |
Y Inhibition (%) |
3.2 10 32 |
0.505 1.000 1.505 |
17 50 73 |
Slope: 55.9637
Intercept: -9.489
Multiple R: 0.9941
N=number of observations: 3
Prediction of x values based on known y values
Known Y Inhibition (%) |
10 xlog (mg/l) |
10x95% (mg/l) |
10x95% (mg/l) |
50 |
11.6 |
0.8 |
157.8 |
Regression line: Y= 55.96 X -9.49
Description of key information
Under the conditions of this present test, HATCOL 5127 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
This concentration was prepared by direct addition of HATCOL 5127 to the test vessels and exceeded the solubility of HATCOL 5127 in water.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
The influence of HATCOL 5127 on the respiration rate of activated sludge was investigated after a contact time of 30 minutes.
The study procedure was based on OECD Guideline No. 209, adopted April 4, 1984 and EEC Directive 67/548 amended November 18, 1987 (87/302), Part C, Publication No. L133, adopted May 30, 1988.
HATCOL 5127 was a light yellow liquid.
Since HATCOL 5127 was poorly soluble in water, the test substance was quantitatively added to the test vessels. A nominal concentration of 100 mg/l was tested in duplicate.
No inhibition of respiration rate of the sludge was recorded at 100 mg HATCOL 5127 per litre. The duplicate measurement confirmed the result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, this study was considered to be valid.
In conclusion, under the conditions of this present test, HATCOL 5127 was not toxic to waste water (activated sludge) bacteria at a concentration of 100 mg/l.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)