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EC number: 259-627-5 | CAS number: 55406-53-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989-03-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: P-2848-8603-P100 - Analytical monitoring:
- yes
- Details on sampling:
- Analysis of the test item for stability over 48 h was conducted in advance of the study. 100 mL of each dilution was allocated for analysis of the test item at the beginning and the end of the study.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: weighed aliquots of the test item were added to Daphnia Synthetic Media and made up to the required volume in glass volumetric flasks. The test item in Daphnia Synthetic Media was dispersed in a sonic water bath for one hour. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Daphnia were bred within the laboratory by acyclical parthenogenesis and the individuals used were between 6 and 24 h old
- Feeding during test: no
ACCLIMATION
- Acclimation conditions: same as test
- Type and amount of food: culture of Chlorella vulgaris and active yeast
- Health during acclimation: no mortality observed - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- approx. 169 mg/L
- Test temperature:
- 20 +/- 2 °C
- Nominal and measured concentrations:
- 5.0, 2.5, 1.25, 0.625, 0.3125 ppm
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Daphnia Synthetic Medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
EFFECT PARAMETERS MEASURED:
Recording of dead daphnids at 0, 3 , 6, 24, and 48 h of exposure
RANGE-FINDING STUDY
- Test concentrations: 1000, 100, 10, 1, 0.1 ppm - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.645 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: equivalent to 0.645 mg/L
- Details on results:
- - Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- LC50 values at 24 and 48 h woth 95 % confidence intervals, were calculated by probit analysis.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The median lethal measured concentration (LC50) of the test item to Daphnia magna has been determined after a 48 h period to be:
48 h LC50 = 0.645 ppm; lower limit: 0.596 ppm; upper limit: 0.696 ppm
Ninety five percent confidence intervals were determined by probit analysis. - Executive summary:
This study was designed to determine the median lethal concentration (LC50) of the test item to Daphnia magna.The study was conducted over a 48 h period under static conditions. Concentrations of 5.0, 2.5, 1.25, 0.625, 0.3125 ppm were applied to four replicates with five daphnids per vessel, respectively. Observations of mortality was performed at 0, 3, 6, 24, and 48 h of exposure. LC50 value with 95 % confidence intervals were calculated with probit analysis. At the two highest concentrations all daphnids died within 48 h of exposure, whereas at the lowest concentration and non-treated control all daphnids survived. LC50 was calulated to be 0.645 ppm.
Reference
Table 1 Determination of acute toxicity to daphnids (48 h static)
Measured Concentration of test item (ppm) |
Number of Mortalities at Time (h) of exposure |
||||
0 |
3 |
6 |
24 |
48 |
|
5.19 |
0 |
0 |
1 |
19 |
20 |
1.94 |
0 |
0 |
0 |
18 |
20 |
0.740 |
0 |
0 |
0 |
0 |
17 |
0.515 |
0 |
0 |
1 |
1 |
1 |
0.448 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Description of key information
In an 48 h acute toxicity study with daphnids under static conditions an LC50 value of 0.645 ppm was determined.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.645 mg/L
Additional information
Acute toxicity to aquatic invertebrates
This study was designed to determine the median lethal concentration (LC50) of the test item to Daphnia magna.The study was conducted over a 48 h period under static conditions. Concentrations of 5.0, 2.5, 1.25, 0.625, 0.3125 ppm were applied to four replicates with five daphnids per vessel, respectively. Observations of mortality was performed at 0, 3, 6, 24, and 48 h of exposure. LC50 value with 95 % confidence intervals were calculated with probit analysis. At the two highest concentrations all daphnids died within 48 h of exposure, whereas at the lowest concentration and non-treated control all daphnids survived. LC50 was calulated to be 0.645 ppm.
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