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Diss Factsheets
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EC number: 907-132-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb 2, 2015 - Feb 5, 2015
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study following ISO Guideline No 10253
- Justification for type of information:
- Read across is based on the category approach. Please refer to attached category document.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Qualifier:
- according to guideline
- Guideline:
- other: OSPAR Guidelines for Toxicity Testing of Substances and Preparations Used and Discharged Off-Shore (reference number: 2002-3, amended March 2005)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,2'-(ethylenedioxy)diethanol
- EC Number:
- 203-953-2
- EC Name:
- 2,2'-(ethylenedioxy)diethanol
- Cas Number:
- 112-27-6
- Molecular formula:
- C6H14O4
- IUPAC Name:
- 2,2'-[ethane-1,2-diylbis(oxy)]diethanol
- Test material form:
- other: clear colorless liquid
- Details on test material:
- - Name of test material (as cited in study report): Triethylene glycol
- Physical state: Clear colorless liquid
- Analytical purity: 99.7% per non-GLP certificate of analysis
- Impurities: tetraethylene glycol 0.3%
- Lot/batch No.: D693EBEA03
- Expiration date of the lot/batch: 11 November 2016
- Storage condition of test material: Room temp in the dark
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
No additional information
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- No additional information.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A nominal amount of test item (100 mg) was dissolved in culture medium and the volume adjusted to 1 liter to give a 100 mg/l stock solution. An aliquot of this stock solution (850 ml) was inoculated with algal suspension (1.6 ml) to give the required test concentration of 100 mg/l. The stock solution and the prepared concentration were inverted several times to ensure adequate mixing and homogeneity.
- Controls: controls were treated identically but not exposed to the test item; culture medium only
- Evidence of undissolved material (e.g. precipitate, surface film, etc): At the start of the test, all control and test cultures were observed to be clear colorless solutions. After the 72 hr test period, all control and test cultures were observed to be brown colored dispersions.
Test organisms
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- - Strain: CCAP 1077/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), Dunstaffnage Marine Laboratory, Oban, Argyll, Scotland
- Culturing media and conditions (same as test or not): Cultures were maintained in the laboratory by the periodic replenishment of culture medium. The culture medium used for the definitive test was the same as that used to maintain the stock culture. The culture was maintained in the laboratory at a temperature of 20 +/- 1 degrees Celsius under continuous illumination (intensity approx. 7000 lux) and constant agitation.
Study design
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- No additional information.
- Test temperature:
- 20 +/- 1 degrees Celsius
- pH:
- 8-8.3
- Dissolved oxygen:
- No additional information.
- Salinity:
- No additional information.
- Nominal and measured concentrations:
- 0, 100 mg/l (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass conical flasks
- Type: plugged with polyurethane foam bungs
- Material, size, headspace, fill volume: 250 ml size; containing 100 ml of test preparation
- Initial cells density: 3x1-e3 cells per ml
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural seawater, sterilized by membrane filtration (mean pore diameter 0.2 micron)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: maemocytometer and light microscope
TEST CONCENTRATIONS
- Test concentrations: 0, 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Total Organic Carbon analysis of the 100 mg/l test preparations containing no algal cells at 0 and 72 hours showed equivalent test item concentrations of 102 and 109 mg/l respectively.
The growth rate of Skeletonema costatum was not affected by the presence of TEG over the 72 hour exposure period. Accordingly, the EC50 (0-72 hr) was >100 mg/l. The NOEC was 100 mg/l.
All test and control cultures were inspected microscopically at 72 hours. There were no abnormalities detected in any of the control or test cultures. - Results with reference substance (positive control):
- A mean growth rate inhibition of 63% was observed with 1.5 mg/l of 3,5-dichlorophenol.
- Reported statistics and error estimates:
- Statistical analysis of the growth rate data was carried out for the control and 100 mg/l test group using a Student's t-test incorporating Bartlett's test for homogeneity of variance (sokal and Rohlf 1981). There were no statistically significant differences (p>/=0.5) between the control and 100 mg/l test group.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The growth rate of Skeletonema costatum was not affected by the presence of TEG over the 72 hour exposure period. Accordingly, the EC50 (0-72 hr) was >100 mg/l. The NOEC was 100 mg/l.
- Executive summary:
The growth rate of Skeletonema costatum was not affected by the presence of TEG over the 72 hour exposure period. Accordingly, the EC50 (0-72 hr) was >100 mg/l. The NOEC was 100 mg/l.
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