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Diss Factsheets
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EC number: 225-555-8 | CAS number: 4926-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
According to the results of the two key studies (Jäger, 2007, Klimisch 1, GLP, OECD 428 & Hadfield, 2005, Klimisch 2, GLP, OECD 428), the test item was considered to have a low dermal penetration rate across human skin. The penetration rate using human skin was defined at 2.01% of the applied dose.
Key value for chemical safety assessment
- Absorption rate - dermal (%):
- 2.01
Additional information
Two key studies were available to assess percutaneous absorption of the test item :
-Jäger, 2007, OECD 428, GLP compliant, Klimisch 1 : This study was performed with the test item, HC Yellow No. 2, at 1.5% in Alkaline Cream Formulation. Two experiments were performed in which porcine ear skin was used in static diffusion cells. 20µL/cm2 of test item was applied in each skin sample, after 30 minutes of incubation, the skin was washed two times. Saline solution was used in receptor chamber. After 24 hours, stratum corneum was isolated by tape stripping, and test item was quantified in receptor chamber using HPLC method. Under the reported conditions, the dermal absorption of HC Yellow 2 was 6.06 ± 2.21 μg/cm2 or 4.71 ± 1.7 % .
-Hadfield, 2005, OECD 428, GLP compliant, Klimisch 2 : The penetration of HC Yellow 2 from a nominal 1% (w/w) formulation, has been measured in vitro through human skin, following the incorporation of [14C]-HC Yellow 2.The formulation was applied to 12 human dermatomed skin membranes , mounted in glass diffusion cells, at a nominal rate of 20mg/cm2. After a contact period of 30 minutes, the dose was washed from the surface of the skin. Samples of the receptor fluid (4% polyoxyethylene 20 oleyl ether solution in phosphate buffered saline) were taken at recorded intervals over a 48h period, during which time the applications remained unoccluded. At the end of the experiment, the surface of the skin was washed again and layers of stratum corneum removed using a tape stripping technique. The receptor fluid samples, sponges, tape strips, residual skin and donor chambers were analysed for radioactivity. Under experimental condition of this study, the results obtained in this study indicated that the penetration of HC Yellow 2 through human skin was very slow, only 2.01% (4.02µg/cm2) of the applied HC Yellow 2 was regarded as systemically available.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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