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EC number: 247-019-2 | CAS number: 25481-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Remarks:
- Experimental data of read across substances
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data for the target chemical is summarized based on the read across chemicals
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- WoE report is based on three short term toxicity to aquatic invertebrates studies as-
WoE 2, WoE 3 and WoE 4. - GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- WoE 3- Concentrations: 0, 25, 50, 100, 200, 400, 800 mg/L
WoE 2, WoE 4: No data available - Vehicle:
- not specified
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Common Name: Water flea
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Test temperature:
- WoE 3: 20 ± 1 °C
- pH:
- WoE 3: 6.8-7.0
- Dissolved oxygen:
- WoE 3: 6.5 mg/L
- Nominal and measured concentrations:
- WoE 2, WoE 4: No data available
WoE 3: 0, 25, 50, 100, 200, 400, 800 mg/L - Details on test conditions:
- WoE 2, WoE 4: No data available
WoE 3: TEST SYSTEM
- Test vessel: Glass Beaker
- Type (delete if not applicable): open
- Volume of solution: 100 ml
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 12
- No. of vessels per concentration (replicates): 4
OTHER TEST CONDITIONS
- Adjustment of pH: 6.8-7.0 with 1 M NaOH
- Photoperiod: 16 hr light with 8 hr darkness
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 - < 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Test chemical was found to be non toxic.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.
- Executive summary:
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
The first study of short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be 113 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
The second study was of short-term toxicity to aquatic invertebrates. The duration of test was 48 hr performed under semi static conditions. Daphnia carinata was taken as a test organism. The test vessel was taken as 100 ml glass beaker. Renewal rate of test solution was done daily. Number of organisms per vessel was taken as 12. Number of vessels per concentration (replicates) was taken as four. Test conditions includes temperature of 20 ± 1 °C, adjustment of pH 6.8-7.0 was done with 1 M NaOH and dissolved oxygen was of 6.5 mg/L. Photoperiod of 16 hr light with 8 hr darkness was provided. Test concentration includes 0, 25, 50, 100, 200, 400, 800 mg/L. Probit analysis was used to calculate the 24-h, 48 hr EC50. On the basis of mortality effect of test chemical on test organism Daphnia carinata the 24, 48 hr EC50 value was determined to be 342.9 mg/L, with 95% CI of 168.5 to 678.6 mg/l and 245.0 mg/L with 95% CI of 200 to 400 mg/l respectively.
The third study includes short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
Reference
Description of key information
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 - < 400 mg/L
Additional information
Data available of the structurally similar read across chemicals has been reviewed to determine the effect of the test chemical on aquatic invertebrates. The studies are as mentioned below:
The first study of short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be 113 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
The second study was of short-term toxicity to aquatic invertebrates. The duration of test was 48 hr performed under semi static conditions. Daphnia carinata was taken as a test organism. The test vessel was taken as 100 ml glass beaker. Renewal rate of test solution was done daily. Number of organisms per vessel was taken as 12. Number of vessels per concentration (replicates) was taken as four. Test conditions includes temperature of 20 ± 1 °C, adjustment of pH 6.8-7.0 was done with 1 M NaOH and dissolved oxygen was of 6.5 mg/L. Photoperiod of 16 hr light with 8 hr darkness was provided. Test concentration includes 0, 25, 50, 100, 200, 400, 800 mg/L. Probit analysis was used to calculate the 24-h, 48 hr EC50. On the basis of mortality effect of test chemical on test organism Daphnia carinata the 24, 48 hr EC50 value was determined to be 342.9 mg/L, with 95% CI of 168.5 to 678.6 mg/l and 245.0 mg/L with 95% CI of 200 to 400 mg/l respectively.
The third study includes short-term toxicity to aquatic invertebrates was carried out. The duration of test was 48 hr performed under static conditions. Daphnia magna was taken as a test organism. On the basis of the immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 mg/l. As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
On the basis of the experimental studies of the structurally similar read across chemical and applying the weight of evidence approach and by evaluating the effect of test chemical on test organism, on the basis of immobility effect of test chemical on daphnia magna the 48 hr EC50 value was determined to be > 100 to < 400 mg/l.As per the value of EC50, the test chemical was considered as not classified as per CLP criteria.
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