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Diss Factsheets
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EC number: 701-293-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Near-guideline, GLP-compliant study. Adequate for assessment.
- Justification for type of information:
- Read across justification included in Section 13
Cross-reference
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- eye irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Near-guideline, GLP-compliant study. Adequate for assessment.
- Justification for type of information:
- Read across justification included in Section 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- Method: Draize Test
- GLP compliance:
- yes
- Species:
- rabbit
- Strain:
- New Zealand White
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- undiluted
Amount applied: 0.1 mL - Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 9
- Details on study design:
- Comment: other: eyes not rinsed in 6 animals and rinsed with water in 3 animals after treatment
- Irritation parameter:
- other: Primary irritation score
- Basis:
- mean
- Time point:
- other: 24 hrs
- Score:
- 4
- Max. score:
- 110
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: Draize scores, unwashed
- Irritation parameter:
- other: primary irritation score
- Basis:
- mean
- Time point:
- other: 48 hr
- Score:
- 1
- Max. score:
- 110
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: draize score, unwashed
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 80
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 10
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.1
- Max. score:
- 8
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.56
- Max. score:
- 12
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Instillation of the material into the conjunctival sac of the rabbit eye, caused only mild transient irritation. All effects had resolved 7 days post exposure
- Executive summary:
Six New Zealand White rabbits were each treated with 0.1 mL of No 6 heavy fuel oil. An additional three animals were also treated with 0.1 ml of test substance, following which their eyes were rinsed with water. Effects on the eyes were monitored over 7 days with scoring according to the Draize scale.
Corneal opacities of grade 1 and area 1 were seen in some animals at the 24 and 48-hour reading. No iridial inflammation was seen in any of the test animals. Conjunctival irritation was seen in all of the rabbits at 24 and 48 hours post dosing. All rabbits were negative for irritation after 7 days. The test substance can be considered mildly irritating. The maximum possible score at each time point is 110.
Corneal opacities of grade 1 and area 1 were seen in three animals at the 24 and 48-hour reading. No iridial inflammation was seen in any of the test animals. Conjunctival irritation was seen in all of the rabbits at 24 and 48 hours post dosing. All rabbits were negative for irritation after 7 days. The test substance can be considered mildly irritating. The maximum possible score at each time point is 110.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- Method: Draize Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 68553-00-4
- Cas Number:
- 68553-00-4
- IUPAC Name:
- 68553-00-4
- Reference substance name:
- No 6 fuel oil
- IUPAC Name:
- No 6 fuel oil
- Test material form:
- other: viscous hydrocarbon liquid
- Details on test material:
- No. 6 Heavy Fuel Oil (API 78-6 with Specific Gravity 0.99/2.7%S),Fuel oil No. 6, CAS No. 68553-00-4.
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- undiluted
Amount applied: 0.1 mL - Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- 9
- Details on study design:
- Comment: other: eyes not rinsed in 6 animals and rinsed with water in 3 animals after treatment
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- other: Primary irritation score
- Basis:
- mean
- Time point:
- other: 24 hrs
- Score:
- 4
- Max. score:
- 110
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: Draize scores, unwashed
- Irritation parameter:
- other: primary irritation score
- Basis:
- mean
- Time point:
- other: 48 hr
- Score:
- 1
- Max. score:
- 110
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: draize score, unwashed
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 80
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 10
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.1
- Max. score:
- 8
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.56
- Max. score:
- 12
- Reversibility:
- fully reversible within: 7 days
- Remarks on result:
- other: unrinsed eye
Any other information on results incl. tables
Corneal opacities of grade 1 and area 1 were seen in three animals at the 24 and 48-hour reading. No iridial inflammation was seen in any of the test animals. Conjunctival irritation was seen in all of the rabbits at 24 and 48 hours post dosing. All rabbits were negative for irritation after 7 days. The test substance can be considered mildly irritating. The maximum possible score at each time point is 110.
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Instillation of the material into the conjunctival sac of the rabbit eye, caused only mild transient irritation. All effects had resolved 7 days post exposure
- Executive summary:
Six New Zealand White rabbits were each treated with 0.1 mL of No 6 heavy fuel oil. An additional three animals were also treated with 0.1 ml of test substance, following which their eyes were rinsed with water. Effects on the eyes were monitored over 7 days with scoring according to the Draize scale.
Corneal opacities of grade 1 and area 1 were seen in some animals at the 24 and 48-hour reading. No iridial inflammation was seen in any of the test animals. Conjunctival irritation was seen in all of the rabbits at 24 and 48 hours post dosing. All rabbits were negative for irritation after 7 days. The test substance can be considered mildly irritating. The maximum possible score at each time point is 110.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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