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EC number: 295-173-4 | CAS number: 91845-03-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12.02.2019 - 13.03.2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- CO 2 Evolution Test", (adopted July 17, 1992).
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and package No.of test material: lot #19953859
- Expiration date of the lot/batch: 16.01.2020 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: Activated sludge from municipal wastewater treatment plant AZV Breisgauer Bucht
- Details on inoculum:
- Activated sludge from the municipal wastewater treatment plant AZV Breisgauer Bucht was used as inoculum with a concentration corresponding to 30 mg dry solids per litre. The treatment plant clarifies predominantly domestic wastewater and has a capacity of 600,000 inhabitant equivalents. Sampling date of activated sludge was on 11 February 2019. The dry solid content of the activated sludge was 4.4 g/L. It was determined by weight measurements after drying at 105°C for 3.25 hours (mean of triplicate measurements). The activated sludge was washed twice with tap water by settling the sludge, decanting the supernatant and re-suspending the sludge.
- Duration of test (contact time):
- >= 28 - <= 28 d
- Initial conc.:
- >= 20 - <= 20 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- In total three reactors containing the test item, three reactors containing only inoculum (blank) and three reactors containing the reference compound were set up. 10.2 mL activated sludge was filled up to 1500 mL with 1489.8 mL mineral medium corresponding to 30 mg/L dry solids. The system was sealed and aerated with CO 2 -free air overnight. The reactors were kept mixed with magnetic stirrers. On the next day, the absorber wash bottles were filled with 0.2 M NaOH and the test substance was added into the test vessels and into the toxicity control vessel. The reference compound was added into the reference vessels and into the toxicity control vessel. The aeration rate was kept at a rate of 30 - 100 mL / min (1.6 - 5.5 bubbles / second) and determined visually daily on working days. The determination by counting the gas bubbles over a defined period using a stop watch was made on days 2 and 27. The CO2 -free air production system, the air-tightness of the whole experimental set-up, the aeration of the absorber flasks and the magnetic stirrers were controlled daily on working days. At the beginning of the study the IC concentration of the 0.2 M NaOH used for the CO2 - absorption flasks was determined as 2.06 mg/L. The IC in the reactors at the beginning of the test was 0.679 mg/L. On the 3rd , 7th, 10th, 14th, 21st and 28th day 4 mL were sampled from the first of two CO 2 -absorber flasks connected in line and the IC's were determined. The vials were
immediately closed with sealing film in order to avoid CO 2 uptake from the air. On the 28th day, sampling of each reactor was performed to measure the CO2 dissolved in water as inorganic carbon (IC). The IC in the second CO2 -absorber flasks, used as protective flasks, was determined on day 29. - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- At the end of the test, a mean degradation extent of 82.9% of ThCO 2 was determined (28 days with considering the IC in the liquid phase, mean of three replicates, see table 1 and figure 1). For finding the exact position of the 10-d window the degradation values of day 3 to 7 and day 10 to 14 were calculated by interpolation. On day 4, the degradation was for the first time greater than 10%. Therefore, the end of the 10-d window is on day 14. On day 14, a mean degradation of 66.7% was calculated. Therefore, the test item reached the criteria for ready biodegradability (60% of ThCO2 within a 10-d window).
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 82.9 - <= 82.9
- St. dev.:
- 1.3
- Sampling time:
- 28 d
- Remarks on result:
- other: readily biodegradable
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance reached the criteria for ready biodegradability (60% of ThCO2 within a 10-d window).
- Executive summary:
At the end of the test, a mean degradation extent of 82.9% of ThCO 2 was determined (28 days with considering the IC in the liquid phase, mean of three replicates, see table 1 and figure 1). For finding the exact position of the 10-d window the degradation values of day 3 to 7 and day 10 to 14 were calculated by interpolation. On day 4, the degradation was for the first time greater than 10%. Therefore, the end of the 10-d window is on day 14. On day 14, a mean degradation of 66.7% was calculated. Therefore, the test item reached the criteria for ready biodegradability (60% of ThCO2 within a 10-d window).
Reference
Description of key information
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- other:
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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