Registration Dossier
Registration Dossier
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EC number: 205-047-2 | CAS number: 132-16-1
Endpoint summary
Administrative data
Description of key information
Biodegradation:
Biodegradation in water:
42-days Closed Bottle test following the OECD guideline 301 D was performed to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as inoculumat concentration 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.The concentration of test and reference item (Sodium Benzoate) chosen for both the study was4 mg/L, while that of inoculum was 32 ml/L. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 79.51 %. Degradation of Sodium Benzoate exceeds 34.33 % on 7 days & 61.44 on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD42 value of test chemical was observed to be 0.35 mgO2/mg. ThOD was calculated as 1.68 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 20.83 %. Based on the results, the test item, under the test conditions, was considered to be primary inherently biodegradable in nature.
Biodegradation in water: simulation tests
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point i.e., biodegradation: simulation testing on ultimate degradation in surface water is technically not feasible and does not need to be conducted since the test chemical is highly insoluble in water.
In additon to this, biodegradation: sediment simulation endpoint is considered for waiver as per in accordance with column 2 of Annex IX of the REACH regulation, the study does not need to be conducted because direct and indirect exposure of the sedime nt compartment is unlikely considering its use as an intermediate in chemical synthesis.
Additional information
Biodegradation:
Biodegradation in water:
42-days Closed Bottle test following the OECD guideline 301 D was performed to determine the ready biodegradability of the test item. The study was performed at a temperature of 20°C. The test system included control, test item and reference item. Polyseed were used as inoculumat concentration 10E7 to 10E8 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism.The concentration of test and reference item (Sodium Benzoate) chosen for both the study was4 mg/L, while that of inoculum was 32 ml/L. OECD mineral medium was used for the study. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (reference item) was also calculated using BOD & ThOD and was determined to be 79.51 %. Degradation of Sodium Benzoate exceeds 34.33 % on 7 days & 61.44 on 14th day. The activity of the inoculum is thus verified and the test can be considered as valid. The BOD42 value of test chemical was observed to be 0.35 mgO2/mg. ThOD was calculated as 1.68 mgO2/mg. Accordingly, the % degradation of the test item after 42 days of incubation at 20 ± 1°C according to Closed Bottle test was determined to be 20.83 %. Based on the results, the test item, under the test conditions, was considered to be primary inherently biodegradable in nature.
Biodegradation in water: simulation tests
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point i.e., biodegradation: simulation testing on ultimate degradation in surface water is technically not feasible and does not need to be conducted since the test chemical is highly insoluble in water.
In additon to this, biodegradation: sediment simulation endpoint is considered for waiver as per in accordance with column 2 of Annex IX of the REACH regulation, the study does not need to be conducted because direct and indirect exposure of the sedime nt compartment is unlikely considering its use as an intermediate in chemical synthesis.
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