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EC number: 233-666-8 | CAS number: 10294-66-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: long-term
- Data waiving:
- other justification
- Justification for data waiving:
- other:
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No standard test, but test well described and relevant ecological endpoints were evaluated (germination,, seedling growth).
- Principles of method if other than guideline:
- Seeds were placed on air-dried soil samples which was moistened with water (control) or water containing the test substance.
The procedure was based on the rules for seed testing published by the Association of Official Seed Analysts. - Analytical monitoring:
- no
- Details on preparation and application of test substrate:
- The soils used were surface (0-15 cm) samples of Iowa soils selected to obtain a range in properties (Webster, Harps, Storden). Each sample was air-dried and crushed to pass through a 2-mm screen
- Species:
- other: corn
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- corn seeds
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 20 degrees celcius
- pH:
- Webster soil: 6.1
Harps soil: 7.7
Storden soil: 8.1 - Moisture:
- 10 mL in 40g dried soil
- Details on test conditions:
- Webster soil: 33 g OC/kg ; 2.4 g total N/kg ; 30% sand, 39% silt, 30% clay
Harps soil: 42 g OC/kg ; 5.0 g total N/kg ; 9% sand, 49% silt, 42% clay
Storden soil: 12 g OC/kg ; 0.6 g total N/kg ; 60% sand, 12% silt, 28% clay
Germination test:
Air-dried soil was placed in a 15 mm x 100 mm Petri Dish and moistened with 10mL of water (control) or with 10 mL of water containing the test compound. Twenty-five seeds of corn were placed on the soil and the Petri dish was covered with a lid and kept in the dark for 7 days in an incubator maintained at 20 °C. The number of seeds germinated was then counted and calculated as a percentage of the number of seeds grown. The criterion for germination was the emergence of a rooting structure and a coleoptile that were longer than the seed. Test was performed in quadruplicate.
Seedling growth test:
20 corn seeds were placed on 40 g of air-dried soil in a 15 mm x 60 mm Petri dish and the soil was moistened with 10 mL of water (control) or with 10 mL of water containing the test compound.
The Petri dish was then covered with a lid and placed in an incubator maintained at 20°C.
After 7 days, the roots and shoots from germinated seeds were measured . All tests reported were performed in triplicate - Nominal and measured concentrations:
- Nominal concentrations of added ATS (mg/kg soil): 0, 100, 500, 1000, 2500, 5000
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: root growth
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: root growth
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Webster soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Webster soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Harps soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Harps soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Storden soil
- Species:
- other: corn
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Storden soil
- Reported statistics and error estimates:
- NOEC represents the highest concentration that resulted in less than 10% effect compared to the control
LOEC represents the highest concentration that resulted in 10% or more effect compared to the control - Conclusions:
- Seeds of corn were germinated on ATS-spiked soil, and effects on germination, root growth and shoot growth were monitored for a 7d-exposure period. Root growth was the most sensitive endpoint, and resulted in a NOEC of 100 mg ATS/kg dry wt. Expressed as thiosulfate, this value becomes 75.7 mg thiosulfate/kg soil. This value can be used for the terrestrial effects assesment of thiosulfates.
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No standard test, but test well described and relevant ecological endpoints were evaluated (germination,, seedling growth).
- Principles of method if other than guideline:
- Seeds were placed on air-dried soil samples which was moistened with water (control) or water containing the test substance.
The procedure was based on the rules for seed testing published by the Association of Official Seed Analysts. - Analytical monitoring:
- no
- Details on preparation and application of test substrate:
- The soils used were surface (0-15 cm) samples of Iowa soils selected to obtain a range in properties (Webster, Harps, Storden). Each sample was air-dried and crushed to pass through a 2-mm screen
- Species:
- other: wheat
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- wheat seeds
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 7 d
- Test temperature:
- 20 degrees celcius
- pH:
- Webster soil: 6.1
Harps soil: 7.7
Storden soil: 8.1 - Moisture:
- 10 mL in 40g dried soil
- Details on test conditions:
- Webster soil: 33 g OC/kg ; 2.4 g total N/kg ; 30% sand, 39% silt, 30% clay
Harps soil: 42 g OC/kg ; 5.0 g total N/kg ; 9% sand, 49% silt, 42% clay
Storden soil: 12 g OC/kg ; 0.6 g total N/kg ; 60% sand, 12% silt, 28% clay
Germination test:
Air-dried soil was placed in a 15 mm x 100 mm Petri Dish and moistened with 10mL of water (control) or with 10 mL of water containing the test compound. Hundred seeds of wheat were placed on the soil and the Petri dish was covered with a lid and kept in the dark for 7 days in an incubator maintained at 20 °C. The number of seeds germinated was then counted and calculated as a percentage of the number of seeds grown. The criterion for germination was the emergence of a rooting structure and a coleoptile that were longer than the seed. Test was performed in quadruplicate.
Seedling growth test:
20 wheat seeds were placed on 40 g of air-dried soil in a 15 mm x 60 mm Petri dish and the soil was moistened with 10 mL of water (control) or with 10 mL of water containing the test compound.
The Petri dish was then covered with a lid and placed in an incubator maintained at 20°C.
After 7 days, the roots and shoots from germinated seeds were measured . All tests reported were performed in triplicate - Nominal and measured concentrations:
- Nominal concentrations of added ATS (mg/kg soil): 0, 100, 500, 1000, 2500, 5000
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: root growth
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: root growth
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Webster soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Webster soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Harps, Storden soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: shoot growth
- Remarks on result:
- other: Harps, Storden soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Remarks on result:
- other: Webster, Harps, Storden soil
- Species:
- other: wheat
- Duration:
- 7 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 2 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- germination
- Remarks on result:
- other: Webster, Harps, Storden soil
- Reported statistics and error estimates:
- NOEC represents the highest concentration that resulted in less than 10% effect compared to the control
LOEC represents the highest concentration that resulted in 10% or more effect compared to the control - Conclusions:
- Seeds of wheat were germinated on ATS-spiked soil, and effects on germination, root growth and shoot growth were monitored for a 7d-exposure period. Root growth was the most sensitive endpoint, and resulted in a NOEC of 100 mg ATS/kg dry wt. Expressed as thiosulfate, this value becomes 75.7 mg thiosulfate/kg soil. This value can be used for the terrestrial effects assesment of thiosulfates.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
Additional information
In a supporting study by McCarty et al. (1990), seeds of corn and wheat were germinated on soil spiked with ammonium thiosulfate, and effects on germination, root growth and shoot growth were monitored for a 7 d-exposure period. Root growth was the most sensitive endpoint, and resulted in a NOEC of 100 mg ammonium thiosulfate/kg dry wt, corresponding to 75.7 mg thiosulfate/kg soil. Results indicate a low potential for the toxicity of thiosulfate substances on seedling germination of terrestrial plants.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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