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EC number: 237-875-5 | CAS number: 14038-43-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://chesar.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
Acute Oral Toxicity:
In Acute oral toxicity, LD50 value for target substance Prussian blue(14068-43-8) was considered to be >8000 mg/kg bw for rats ; >8000 mg/kg bw for mouse and >8000 mg/kg bw for rabbits. All these studies concluded that the LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Prussian blue(14068-43-8) cannot be classified for acute oral toxicity.
Acute Inhalation Toxicity:
The melting point of C.I.Pigment Blue 27 is >300 C. This suggests that Prussian blue insoluble decomposes between 210°C -360 °C without melting and does not exhibit very high vapour pressure. Inhalation is therefore not likely route of exposure and hence this end point is considered for waiver.
Acute Dermal Toxicity:
The Prussian blue insoluble (14038-43-8) has high molecular weight i.e 877.381g/mole. This suggests that Prussian blue insoluble has no potential for significant rate of absorption through skin and hence this end point is considered for waiver.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative database
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute oral toxicity study of Prussian blue(14068-43-8) was performed in Rat
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
- Specific details on test material used for the study:
- - Name of test material: Prussian blue
- IUPAC name: C.I.Pigment Blue 27
- Molecular formula: C6FeN6.4/3Fe
- Molecular weight (if other than submission substance): 859.23 g/mol
- Smiles : [CH-](#N)[Fe+2]([CH-]#N)([CH-]#N)([CH-]#N)([CH-]#N)[CH-]#N.[CH-](#N)[Fe+2]([CH-]#N)([CH-]#N)([CH-]#N)([CH-]#N)[CH-]#N.[CH-]
(#N)[Fe+2]([CH-]#N)([CH-]#N)([CH-]#N)([CH-]#N)[CH-]#N.[Fe+3].[Fe+3].[Fe+3].[Fe+3]
- Inchi : 1S/18CN.7Fe/c18*1-2;;;;;;;/q18*-1;3*+2;4*+3
- Substance type: Inorganic
- Physical state: Solid powder (dark blue) - Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- No data available
- Route of administration:
- oral: unspecified
- Vehicle:
- not specified
- Details on oral exposure:
- No data available
- Doses:
- 8000 mg/kg bw
- No. of animals per sex per dose:
- No data available
- Control animals:
- not specified
- Details on study design:
- No data available
- Statistics:
- No data available
- Preliminary study:
- No data available
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- > 8 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No mortality was observed
- Mortality:
- No mortality was observed at dose 8000 mg/kg bw
- Clinical signs:
- other: No data available
- Gross pathology:
- No data available
- Other findings:
- No data available
- Interpretation of results:
- other: Not classified
- Conclusions:
- the LD50 value was considered to be >8000 mg/kg body weight,when rats was treated with Prussian blue (14038-43-8) orally.
- Executive summary:
Acute oral toxicity study was done in rats using Prussian blue (14038-43-8).No mortality was observed at dose 8000mg/kg bw.Hence LD50 was considered to be >8000mg/kgbody weight,when rats were treated with Prussian blue (14038-43-8)orally.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 8 000 mg/kg bw
- Quality of whole database:
- Data is Klimisch 2 and from authoritative database
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Data waiving:
- other justification
- Justification for data waiving:
- other:
Reference
Endpoint conclusion
- Quality of whole database:
- Waiver
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Data waiving:
- other justification
- Justification for data waiving:
- the study does not need to be conducted because the physicochemical and toxicological properties suggest no potential for a significant rate of absorption through the skin
Reference
Endpoint conclusion
- Quality of whole database:
- Waiver
Additional information
Acute Oral Toxicity:
In different studies, Prussian blue insoluble (14038-43-8) has been investigated for acute oral toxicity to a greater or lesser extent. Often the studies are based on in vivo experiments data in rodents, i.e. most commonly in rats for Prussian blue insoluble (14038-43-8). The different studies on target substance are summarized as below –
In another experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance Prussian blue insoluble (14038-43-8). Acute oral toxicity study was done in rats using Prussian blue (14038-43-8).No mortality was observed at dose 8000mg/kg bw.Hence LD50 was considered to be >8000 mg/kg body weight,when rats were treated with Prussian blue (14038-43-8)orally.
Also these results are further supported by the experimental study conducted by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance Prussian blue insoluble (14038-43-8). Acute oral toxicity study was done in mouse using Prussian blue (14038-43-8).No mortality was observed at dose 8000 mg/kg bw.Hence LD50 was considered to be >8000mg/kg body weight,when mouse was treated with Prussian blue (14038-43-8)orally.
This is further supported by U.S. National Library of Medicine (Chemidplus Database,U.S. National Library of Medicine,2017) for the target substance Prussian blue insoluble (14038-43-8). Acute oral toxicity study was done in rabbit using Prussian blue (14038-43-8).No mortality was observed at dose 8000 mg/kg bw.Hence LD50 was considered to be >8000 mg/kg body weight,when rabbit was treated with Prussian blue (14038-43-8)orally.
Thus, based on the above different studies on Prussian blue insoluble (14038-43-8), it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Prussian blue insoluble (14038-43-8)cannot be classified for acute oral toxicity.
Acute Inhalation Toxicity:
The melting point of C.I.Pigment Blue 27 is >300 C. This suggests that Prussian blue insoluble decomposes between 210°C -360 °C without melting and does not exhibit very high vapour pressure. Inhalation is therefore not likely route of exposure and hence this end point is considered for waiver.
Acute Dermal Toxicity:
The Prussian blue insoluble (14038-43-8) has high molecular weight i.e 877.381g/mole. This suggests that Prussian blue insoluble has no potential for significant rate of absorption through skin and hence this end point is considered for waiver.
Justification for classification or non-classification
Based on the above experimental studies on Prussian blue insoluble (14038-43-8), it can be concluded that LD50 value is greater than 2000 mg/kg bw. Thus, comparing this value with the criteria of CLP regulation, Prussian blue insoluble (14038-43-8) cannot be classified for acute oral toxicity. For Acute dermal and acute inhalation toxicity wavier was added so, not possible to classify.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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