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EC number: 248-315-4 | CAS number: 27194-74-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 November 2016 to 19 January 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Physical state/Appearance: Clear colorless liquid
Batch: 1023R25811
Purity: 99.5%
Expiry Date: 14 September 2019
Storage Conditions: Room temperature in the dark - Analytical monitoring:
- yes
- Remarks:
- gas chromatography
- Details on sampling:
- The test samples were thawed with the aid of a water bath. The test samples (200 mL) plus 60 g of sodium chloride were extracted directly with 20 mL of hexane in the same bottle. An aliquot of the hexane layer was taken for analysis.
- Vehicle:
- no
- Details on test solutions:
- A nominal amount of test item was dispersed in culture medium with the aid of propeller stirring. After 24 hours the stirring was stopped and any undissolved test item was removed by filtration through a 0.2 µm filter (first approximate 2 liters discarded in order to precondition
the filter) to give a 100% v/v saturated solution.
The concentration and stability of the test item in the test preparations were verified by chemical analysis at 0, 24, 48 and 72 hours. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: CCAP 278/4
- Method of cultivation: flask under constant agitation by orbital shaker, constant aeration and constant illumination - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 ± 1 °C
- pH:
- 7.7 - 8.4
- Nominal and measured concentrations:
- 6.25, 12.5, 25, 50 and 100% v/v saturated solution.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flasks
- Type: closed
- Material, size, headspace, fill volume: 250 mL glass conical flasks
- Aeration: continuous
- Initial cells density: 5.00 x 103 cells/mL
- No. of vessels per concentration (replicates): 5.00 x 103 cells/mL
- No. of vessels per control (replicates): 5.00 x 103 cells/mL
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: yes
- Photoperiod: continuous
- Light intensity and quality:7000 lux warm white lighting (380 – 730 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.10, 1.0, 10 and 100% v/v saturated solution
- Results used to determine the conditions for the definitive study: The results showed no effect on growth at the test concentrations of 0.10, 1.0 and 10% v/v saturated solution. However, growth was observed to be reduced at 100% v/v saturated solution.
Based on this information test concentrations of 6.25, 12.5, 25, 50 and 100% v/v saturated solution were selected for the definitive test. - Reference substance (positive control):
- yes
- Remarks:
- The positive control was conducted between 28 November 2016 and 01 December 2016.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.068 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.033 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.047 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.051 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.033 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.047 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- biomass
- Details on results:
- All test and control cultures were inspected microscopically at 72 hours. After 72 hours there were no abnormalities detected in the control or test cultures at 0.033 and 0.047 mg/L, no intact cells were observed to be present in the test cultures at 0.060, 0.077 and 0.94 mg/L
- Results with reference substance (positive control):
- ErC50 (0 – 72 h) : 1.4 mg/L; 95% confidence limits 1.2 – 1.5 mg/L
EyC50 (0 – 72 h) : 0.60 mg/L; 95% confidence limits 0.52 – 0.69 mg/L
The results from the positive control with potassium dichromate were within the normal ranges for this reference item. - Reported statistics and error estimates:
- One way analysis of variance incorporating Bartlett's test for homogeneity of variance and Dunnett's multiple comparison procedure for comparing several treatments with a control was carried out on the growth rate and yield data after 72 hours for the control and all test concentrations to determine any statistically significant differences between the test and control groups.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and based on the geometric mean measured test concentrations:
Growth rate:
- EC50 = 0.068 mg/L ; 95% Confidence Limits = 0.064 - 0.072 mg/L
- No Observed Effect Concentration (NOEC) = 0.033 mg/L
- Lowest Observed Effect Concentration (LOEC) = 0.047 mg/L
Yield:
- EC50 = 0.051 mg/L ; 95% Confidence Limits = 0.049 - 0.054 mg/L
- No Observed Effect Concentration (NOEC) = 0.033 mg/L
- Lowest Observed Effect Concentration (LOEC) = 0.047 mg/L
Reference
Description of key information
Toxicity to Pseudokirchneriella subcapitata over a 72-Hour period:
Growth rate:
- EC50 = 0.068 mg/L ; 95% Confidence Limits = 0.064 - 0.072 mg/L
- No Observed Effect Concentration (NOEC) = 0.033 mg/L
- Lowest Observed Effect Concentration (LOEC) = 0.047 mg/L
Yield:
- EC50 = 0.051 mg/L ; 95% Confidence Limits = 0.049 - 0.054 mg/L
- No Observed Effect Concentration (NOEC) = 0.033 mg/L
- Lowest Observed Effect Concentration (LOEC) = 0.047 mg/L
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.068 mg/L
- EC10 or NOEC for freshwater algae:
- 0.033 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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