3-methoxy-N,N-dimethylpropionamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 September 2011 - 10 February 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Version / remarks:

1996

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: SEPA (HJ/T 153-2004) 'The guidelines for testing of chemicals'

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: "The guidelines for the testing of chemicals", Beijing: China Environmental Sciences Publishing House. SEPA.

Version / remarks:

2004

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 0, 100 and 150 mg/L
- Sampling method: samples were taken at least in duplicate from all concentrations during the limit test at 0, 24, 48, 72 and 96 h. On each occasion, one sample was analysed. Remaining samples were retained in case further analysis would be required.
- Sample storage conditions before analysis: samples were abalysed directly after collection and pre-treatment.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test solutions were prepared by directly dissolving the appropriate amount of test item in dilution medium. Dispersion was facilitated by ultrasonication for 20 minutes.
- Controls: yes, test medium without any additives was used as a control group (blank-control)

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Batch No.: F20111009
- Source: obtained from a commercial fish supplier of Guangzhou Hongfa Aquatic Animals Culture Company, China, 21 days before the start of the test.
- Age at study initiation (mean and range, SD): not indicated
- Length at study initiation: 2.30 cm (mean)
- Weight at study initiation (wet weight): 0.20 g (mean)

ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions: in holding tanks with a continuous flow of aerated water of the quality and temperature used in the test.
- Feeding during acclimation: daily, with proprietary fish food up until 24 hours before being placed into the test vessels.
- Health during acclimation (any mortality observed): after the 48-hour settling-in period, no mortality was observed in the following 7 days.

FEEDING DURING TEST: no

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

119 - 126 mg CaCO3/L throughout the test

Test temperature:

23.0 - 23.4 throughout the test

pH:

7.7 - 7.9 throughout the test

Dissolved oxygen:

Oxygen concentration: 87% - 99% of air saturation throughout the test

Nominal and measured concentrations:

Nominal concentrations (limit test): 100 and 150 mg/L
Mean measured concentrations (limit test): 96.3 and 142 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 8 L tanks made of chemically inert material, with a sealable inert lid
- Fill volume: 5 L
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.2-0.6 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated (for at least 24 hours) tap water
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH, dissolved oxygen and temperature were measured daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours daily
- Light intensity: 1000 - 1500 lux

EFFECT PARAMETERS MEASURED: mortality at 24, 48, 72 and 96 hours

RANGE FINDING STUDY
- Test concentrations: 0, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate (July 2011)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 150 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- No mortality was observed in the control group or any of the treatment groups, therefore the effect concentration was determined to exceed the highest tested concentration.
- No behavioural or biological abnormalities were observed.
- Analytical results showed that test concentrations remained stable during the exposure period (96% and 95% of nominal concentrations), therefore the effect concentration was based on nominal concentrations.
- pH, temperature and dissolved oxygen concentrations were maintained throughout the exposure period.

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: 100% after 18 hours at the highest concentration (500 mg/L)
- 24h-LC50: 262 mg/L
- Other: results are given for a 24 hour test period

Reported statistics and error estimates:

Trimmed Spearman-Karber Method (Version 1.5, USEPA) were used to calculate the LC50 and 95% confidence limits of the reference substance (no LC50 and confidence limits could be calculated for the test item due to its low toxicity to fish).

Validity criteria fulfilled:

yes

Remarks:

No mortality was observed in the control groups, test conditions were maintained during the test, dissolved oxygen concentrations were >60% (i.e., 87-99%) and analysis showed that test concentrations remained stable during the exposure period.

Conclusions:

Under the conditions of the test, the 96h-LC50 of Equamide M100 to zebra fish was >150 mg/L (nominal concentration).

Executive summary:

The acute toxicity to fish of Equamide M100 was assessed in a study according to OECD 203 under static conditions. 10 zebra fish (Brachydanio rerio) per concentration and control group were exposed to the test item for 96 hours in two limit tests. Three replicates were used per concentration and for the control group. Nominal concentrations were 100 and 150 mg/L. Test concentrations were analytically verified to be 96.3 mg/L and 142 mg/L and therefore showed to remain stable during the exposure period (within 20% of initial test concentrations). No mortality was observed in the control groups or any of the treatment groups. Therefore, the 96h-LC50 was determined to exceed the highest tested concentration (> 150 mg/L). All acceptability criteria were met and the study was considered to be valid.

Description of key information

The acute toxicity to fish of Equamide M100 was assessed in a study according to OECD 203 under static conditions. 10 zebra fish (Brachydanio rerio) per concentration and control group were exposed to the test item for 96 hours in two limit tests. Three replicates were used per concentration and for the control group. Nominal concentrations were 100 and 150 mg/L. Test concentrations were analytically verified to be 96.3 mg/L and 142 mg/L and therefore showed to remain stable during the exposure period (within 20% of initial test concentrations). No mortality was observed in the control groups or any of the treatment groups. Therefore, the 96h-LC50 was determined to exceed the highest tested concentration (> 150 mg/L). All acceptability criteria were met and the study was considered to be valid.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

150 mg/L