magnesium bis(dipropan-2-ylazanide)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Principles of method if other than guideline:

Deviations compared with the guideline:
The temperature range was higher than ± 1°C as stated in the guidelines. As no mortality occurred in the control, this deviation was stated as uncritical
No analytical determination of the content of the test item in the test solutions during the test was made as no analytical method for the measurement of the test item in the test medium could be developed.
The highest variation of the pH-value in the test was higher than the demanded variation in the EU-guideline (1.2 units in the treatment 32 mg/L). As this variation is slightly higher than allowed and it was probably caused by the test items properties, this was stated as uncritical.

GLP compliance:

yes (incl. QA statement)

Test material

Reference

Name:

Unnamed

Type:

Constituent

Type:

Constituent

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

31 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The study results for the test item MDA were as follows: NOEC (96h) = 18 mg/L and LC50 (96h) = 31 mg/L.

Executive summary:

The determination of the acute toxicity of MDA following EU-Method C.1 resp. OECD Guideline 203 was performed against Danio rerio HAMILTON BUCHANAN over 96 h under static conditions with nominal concentrations of 0, 10, 18, 32, 56, and 100 mg MDA/L. The study results for the test item MDA were as follows: NOEC (96h) = 18 mg/L and LC50 (96h) = 31 mg/L.