Nickel, compound with Titanium (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2013

Report date:

2013

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Nickel, compound with Titanium (1:1)

EC Number:

610-765-8

Cas Number:

52013-44-2

Molecular formula:

NiTi

IUPAC Name:

Nickel, compound with Titanium (1:1)

Details on test material:

100% Equiatomic NiTi intermetallic compound
Batch 1048

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

The test item has been prepared as a stck solution (10 g/l) and then this solution and 4 different concentrations in water (max solubility) have been tested. Because the test item is poorly soluble in water to facilitate its solubility in water the stock solution has been stirred for 70 hours and then before the beginning of the test this solution has been ultrasonicated for 30 minutes.
The total volume of the culture medium and the concentration of the algae inoculum added are the same in all vessels.

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Family: chlorophyceae
- order: chlorococcalles
- culturing: the algal suspension has been kept in culture medium in sterile 250 ml Erlenmeyer flasks at 23 +/- 2 °C, with lighting greater than 4300 lux and steady with electrical stirrer

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

Every 24 hours from the beginning of the test until the end of the test (72 hours), cellular concentration has been determined, by means of Burker chamber, of treated and of control organisms.

Test conditions

Test temperature:

23 +/- 2 °C

pH:

from 7.70 to 9

Nominal and measured concentrations:

Administered solutions (g/l): 10.00, 3.130, 0.980, 0.310, 0.095

Details on test conditions:

Because the test will done on the extract of the test item, constituted by metals with a low volatilisation from water to air, at the beginning and at the end of the test have been checked the concentrations of two metals (nichel and titanium) in the extract by ICP-OES.

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 g/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

A normal and healthy appearance of the algae at the end of the test was observed at microscopic.

Any other information on results incl. tables

Algal density (cells/ml) in the treated group 10 g/l
Time (days)	1	2	3
0	10000	10000	10000
1	32000	33000	32000
2	260000	240000	210000
3	630000	650000	620000

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The obtained results after 72 hours showed that EC50 of the test item is greater than 10 g/l.