Reaction products of 4-amino-3-hydroxy-7-nitronaphthalene-1-sulfonic acid and 2′,4′-dihydroxyacetophenone chelated with iron, sodium salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Source study has reliability 2. Details on the read across are available in section 13.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: an aqueous solution (1% w/v) of substance was added to test medium.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: West Aquarium GmbH, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (range: 2.4 - 3.4 cm)
- Weight at study initiation (mean and range, SD): 0.26 g

ACCLIMATION
- Type and amount of food: TETRA MIN, standard feed for aquarium fish, Tetra-Werke, Melle, Germany; ad libitum.
- Feeding frequency: daily.
- Health during acclimation (any mortality observed): no mortality observed.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23 - 25°C

pH:

7.8 - 8.1

Dissolved oxygen:

7.4 - 8 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
6.81, 10.00, 14.70, 21.50, 31.60, 46.40, 68.10, 100.00 mg/l

Analytically determined concentrations:
5.10, 7.80, 11.40, 16.90, 24.00, 35.70, 51.00 and 76.60 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium ( 30 × 22 × 24 cm)
- Fill volume: 10 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.26 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater according to DIN 38412 part 11

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptoms of intoxication after 1, 24, 48, 72 and 96 hours.

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

20.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Gasping and tumbling were observed. Since test solutions were intensly coloured, symptoms could not be observed in more detail.

Reported statistics and error estimates:

FINNEY, D.J., PROBIT ANALYSIS, CAMBR. UNIV. PRESS, 3RD ED., 1971

Conclusions:

LC50 (96h) = 20.7 mg/l.

Executive summary:

Method:

Acute toxicity to fish was assessed using nominal test concentrations of 6.81, 10.0, 14.7, 21.5, 31.6, 46.4, 68.1, 100 mg/l. Ten fish per concentration were exposed under static conditions for up to 96 h. Effects in terms of mortality were recorded at 1, 24, 48, 72 and 96 h after start.

Results:

LC50 (96h) = 20.7 mg/l

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Source study has reliability 2. Details on the read across are available in section 13.

Principles of method if other than guideline:

Test method described in JIS K 0102 - 1974-ITEM 55.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: the substance was added to test medium without any pretreatment.

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM
- Common name: red Killifish.
- Source: bred at testing facility.
- Length at study initiation (length definition, mean, range and SD): 3.0 cm (range: 2.8 - 3.4 cm).
- Weight at study initiation (mean and range, SD): 0.26 g.

ACCLIMATION
- Type and amount of food: TETRA MIN, standard feed for aquarium fish, Tetra-Werke, Melle, Germany; ad libitum.
- Feeding frequency: daily.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

26°C

pH:

8.2 - 8.6

Dissolved oxygen:

7.3 - 8.1 mg/l

Nominal and measured concentrations:

Nominal test substance concentrations:
46.4, 100, 215, 464 and 1000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: glass aquarium ( 30 × 22 × 24 cm)
- Fill volume: 5 l
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.5 g fish/l

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water, filtered by carbon filter

OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and symptomas of intoxication after 24, 48 and 96 hours.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.2
- Results used to determine the conditions for the definitive study: LC50 after 96 h: ca. 250 mg/l

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 46.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

181 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Symptoms of intoxication were not observed.

Reported statistics and error estimates:

FINNEY, D.J., PROBIT ANALYSIS, CAMBR. UNIV. PRESS, 3RD ED., 1971

Conclusions:

LC50 (96h) = 181 mg/l

Executive summary:

Method:

Acute toxicity to fish was assessed using nominal test concentrations of 46.4, 100, 215, 464 and 1000 mg/l. Ten fish per concentration were exposed under static conditions for up to 96 h. Effects in terms of mortality were recorded at 0, 24, 48 and 96 h after start.

Results:

LC50 (96h) = 181 mg/l.

Description of key information

LC₅₀ (96h) freshwater, Zebra fish = 20.7 mg/l

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

20.7 mg/L

Additional information

No data on target substance was available, thus a read across approach was followed, using data on Similar Substance 01. Details are reported in section 13.

Short term toxicity to fish was assessed in 2 studies on Similar Substance 01, under static exposure conditions and exposure duration of 96 h, using 2 different species. Both studies were well-documented and reliable, thus the study reporting the lowest LC50 value was used for classification, following a precautionary approach.

In a study conducted according to OECD guideline 203 on Danio rerio species, nominal tested concentrations ranged from 6.81 to 100 mg/l. One replicate with 10 fish per concentration was tested. Effects of mortality were recorded at 1, 24, 48, 72 and 96 h after start of exposure. LC50(96h) of 20.7 mg/l was found.

In a study conducted on the species Oryziaz latipes, concentrations in the range of 46.1 up to 1000 mg/l were used. After 96 h, a LC50 of 181 mg/l was found.