4-isopropylaniline

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Principles of method if other than guideline:

Guideline 67/548/EWG (Acute toxicity to daphnia, C.2)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-isopropylaniline

EC Number:

202-797-2

EC Name:

4-isopropylaniline

Cas Number:

99-88-7

Molecular formula:

C9H13N

IUPAC Name:

4-(propan-2-yl)aniline

Details on test material:

- Name of test material (as cited in study report): p-isopropylanilin
- Analytical purity: 99.8
- Lot/batch No.: 04012348 (Tank 505)

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Preparation of stock solution with 120 mg/L. Dilution series with factor radical 10.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Waterflea
- Strain: Daphnia magna STRAUS
- Source: Original from German health authority (BGA), Berlin, than breed in laboratory of Bayer AG, Leverkusen
- Age at study initiation: 0-24 h

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

13.5 °dH

Test temperature:

19.5 ± 0.3 °C

pH:

7.8 - 7.9

Dissolved oxygen:

9.3 - 9.6 mg/L

Nominal and measured concentrations:

Nominal concentrations: Control, 0.3, 1.0, 3.2, 10.0, 31.6, 100 mg/L
Measured concentrations(Initial): < 2, not detectable, not detectable, 3.0, 9.0, 24.0, 74.0 mg/L TOC
Measured concentrations(48h): 3, not detectable, not detectable, 4.0, 8.0, 24.0, 71.0 (after 24h) mg/L TOC
measured concentrations refers to TOC, 1 mg/L TOC equates to 1.3 mg/L test substance

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: cylindrical beaker (diameter 4 cm, high: 6.5 cm), 20 ml test medium
- Aeration: no

- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium (Elendt)

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

0.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

other: EC90

Effect conc.:

3.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 4

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

2.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Nominal conc.(mg/l)	Immobile daphnids after 24 h	Immobile daphnids after 48h
0.3	0	0
1.0	5	13
3.2	15	18
10.0	15	19
31.6	20	20

Applicant's summary and conclusion

Executive summary:

A test on the acute toxicity of 4 -isopropylaniline to the invertebrate Daphnia magna was performed according to the Guideline 67/548/EWG (Acute toxicity to daphnia, C.2) in a static test system. For a test period of 48 hours an EC50 value of 0.9 mg/l based on nominal concentrations was reported (Bayer AG, 1997).