Cyclododecanone, 2-(2-methyl-2-propen-1-yl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GPL guideline study without restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-(2-methylprop-2-en-1-yl)cyclododecan-1-one

EC Number:

691-657-8

Cas Number:

62599-49-9

Molecular formula:

C16 H28 O

IUPAC Name:

2-(2-methylprop-2-en-1-yl)cyclododecan-1-one

Details on test material:

- Name of test material (as cited in study report): 2-(2-Methylallyl)-cyclododecanon-Rein
- Physical state: colorless, clear liquid
- Analytical purity: 99.3 area % (not corrected with the water content)
- Expiration date of the lot/batch: October, 2014
- Stability under test conditions: stable
- Water solubility: 1.4 mg/L at 20 °C.
- Storage condition of test material: at ambient temperature without exposure to light in a tightly sealed container

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations : 1.4, 0.7, 0.35, 0.18, 0.09 mg/L and control (nominal)
- Sampling method: The aim of the analytical part of the definitive test was to determine the concentrations of 2-(2-
Methylallyl)-cyclododecanon-Rein using a validated gas chromatography method with FID detection
[SOP/C/289]. In the definitive test, the samples at exposure initiation and at exposure termination collected from
each test item concentration and the control were analyzed [SOP/W/83].
- Sample storage conditions before analysis: The sample of the test item was stored at ambient temperature without exposure to light in a tightly
sealed container [SOP/W/3, SOP/PB/1].

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
In the definitive test following test item concentrations were used: 1.4, 0.7, 0.35, 0.18 and 0.09 mg/L
plus the control (with a spacing factor of 2.0). The 3 μL of the test item was added into 200 mL of
Elendt M7 medium in glass flask with a capacity of 250 mL using a glass syringe (Hamilton, 10 μL,
Switzerland). The content of flask was thoroughly mixed and, then content of flask was transferred into
glass flask with a capacity of 5 L by multiple washing with Elendt M7 medium. The glass flask was
filled up to 2 L of Elendt M7 medium. The content of flask was ultarsonicated for 5 minutes
[SOP/W78]. After that the test item concentration of 1.4 mg/L was visually transparent and
homogeneous. The test item was visually dissolved. The lower test item concentrations were prepared
by sequential dilutions with the Elendt M7 medium in a ratio of 1 : 1 (volume per volume).
The control (550 mL of Elendt M7 medium) was ultarsonicated for 5 minutes [SOP/W78].

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Source: Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology.
- Age at study initiation (mean and range, SD): < 24 h old at exposure initiation); not first brood progeny.
- Method of breeding: Daphnia magna was cultured in glass beakers with a capacity of 150 mL (one parent per vessel)
containing 100 mL of Elend M7 medium, at room temperature ranging from 18 to 22°C with daily cycle 16 h light : 8 h dark.
- Feeding during test: no feeding.

ACCLIMATION
- Type and amount of food: suspension of algae; mixture of two species Pseudokirchneriella subcapitata: Desmodesmus subspicatus (in 2:1 ratio) Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilized suspension of Spirulina sp.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.0 – 21.0 ºC

pH:

7.52 – 7.53

Dissolved oxygen:

8.9 – 9.6 mg/L

Nominal and measured concentrations:

Nominal concentrations: 1.4, 0.7, 0.35, 0.18, 0.09 mg/L and control.
Measured concentrations: 1.3, 0.6, 0.32, 1.17, 0.08 and control.

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers of 150 mL (with transparent lids)
- Type : closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
The Elendt M7 medium recommended by the OECD Guideline No. 202 (2004) was used to culture the test organism and as a diluent/solvent of the test item.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daily cycle 16 h light : 8 h dark; fluorescent light source

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Daphnia magna was observed for immobilization after 24 and 48 h of exposure. The Daphnia magna
was considered immobile if they showed no ability to swim within 15 seconds after swirling the test
vessel.
- The temperature was continuously recorded.
- The pH values and the dissolved oxygen concentrations were measured at exposure initiation, i.e.
before the split up into replicates, and at exposure termination in pooled replicates

TEST CONCENTRATIONS
- Range finding study: preliminary test
- Results used to determine the conditions for the definitive study: were determined on the basis of the preliminary tests results

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.48 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limits: 0.41 – 0.56 mg/L).

Effect concentrations 2

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

0.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.35 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

- EC50: 0.60 mg/L

Reported statistics and error estimates:

- Statistical analysis: Probit method calculations and analysis by Fisher’s Exact Binomial Test with Bonferroni Correction.

Any other information on results incl. tables

S ince the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.