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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to a test protocol that appears to be comparable to the appropriate OECD test guideline. There is no information on GLP compliance.

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
GLP compliance:
not specified
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Furan
EC Number:
203-727-3
EC Name:
Furan
Cas Number:
110-00-9
Molecular formula:
C4H4O
IUPAC Name:
furan
Details on test material:
- Name of test material (as cited in study report): Furan
- Molecular weight: 68.08
- Substance type: cyclic, dienic ether
- Physical state: clear, colourless liquid
- Analytical purity: 99%

Test animals

Species:
mouse
Strain:
B6C3F1
Sex:
male

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
corn oil
Duration of treatment / exposure:
Single dose
Frequency of treatment:
Once
Post exposure period:
bone marrow harvest: 17 h first experiment, 36 h second experiment
Doses / concentrations
Remarks:
Doses / Concentrations:
87.5, 175, 350 mg/kg bw
Basis:

No. of animals per sex per dose:
10
Control animals:
yes, concurrent vehicle
Positive control(s):
- :Positive control: dimethylbenzanthracene
- Justification for choice of positive control(s): none given
- Route of administration: subcutaneous implantation
- Doses / concentrations: 100 mg/kg bw

Examinations

Tissues and cell types examined:
Bone marrow cells
Details of tissue and slide preparation:
CRITERIA FOR DOSE SELECTION: based on pre-experiment for toxicity

DETAILS OF SLIDE PREPARATION: fixation, drying and staining

METHOD OF ANALYSIS: 50 first division metaphase cells scored from 8 animals per treatment
Statistics:
Trend test (Margolin et al 1986)

Results and discussion

Test results
Sex:
male
Genotoxicity:
positive
Toxicity:
not specified
Vehicle controls validity:
valid
Negative controls validity:
not applicable
Positive controls validity:
valid

Any other information on results incl. tables

Induction of chromosomal aberrations in mouse bone marrow cells by Furan

Trial 1 - 17 hour harvest time

Treatment mg/kg

% Cells with aberrations

Solvent control

2.0 ± 0.01

87.5

1.0 ± 0.01

175.0

1.0 ± 0.00

350.0

2.3 ± 0.01

Positive control

17.0 ± 0.07

 

Trial 2 - 36 hour harvest time

Treatment mg/kg

% Cells with aberrations

Solvent control

1.43 ± 0.01

62.5

3.5 ± 0.01*

125.0

3.25 ± 0.01

250.0

16.75 ± 0.06***

Positive control

27.5 ± 0.10

 

 Trial 3 - 36 hour harvest time

Treatment mg/kg

% Cells with aberrations

Solvent control

1.75 ± 0.01

62.5

3.5 ± 0.01

125.0

3.0 ± 0.00

250.0

19.5 ± 0.06***

Positive control

18.0 ± 0.06

 

 * = Positive (P ≤0.05)

*** = Positive (P ≤0.001)

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): positive
Significant increases in micronucleus frequency were observed in mouse bone marrow cells after ip administration of furan; the effect was observed only after extended harvest time at highest dose tested (250 mg/kg bw).