Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 929-513-6 | CAS number: 1400584-86-2
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- 2001
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
- Version / remarks:
- 2001
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
Test material
- Reference substance name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- EC Number:
- 929-513-6
- Cas Number:
- 1400584-86-2
- Molecular formula:
- C9H13N2O3 · Na
- IUPAC Name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- Test material form:
- solid: bulk
Constituent 1
- Radiolabelling:
- no
Study design
- Test temperature:
- 25 °C
HPLC method
- Details on study design: HPLC method:
- - HPLC equipment: Agilent HPLC system with autosampler G1313A, pump G1312A and column oven G1316A, UV/VIS detector G1314A; Software Chromeleon Vers. 6.70, Fa. Dionex
- Detection wavelength: 254 nm (calibration graph and thiourea), 210 nm (analysis of the test item and thiourea)
- Column: Material: stainless steel (Bischoff), dimensions: 250 x 4.0 mm, stationary phase: Zorbax CN, 5 µm, column temperature: 25 °C
- Mobile Phase: pH 2: 0.01 M phosphoric acid / acetonitrile 60/40 (v/v) adjusted to pH 2 with phosphoric acid
pH 9: 0.01 M phosphoric acid / acetonitrile 60/40 (v/v) adjusted to pH 9 with phosphoric acid and sodium hydroxide (isocratic elution)
- Injected volume: 10 µL
- Flow rate: 1.0 mL/min
- Injection amounts:
Calibration substances: 0.11 -18 µg; Test item: 0.51 µg; Thio urea: 0.2 µg
The calibration substances were injected in duplicate dissolved in acetonitrile and the test item was injected in duplicate dissolved in acetonitrile/water 80/20 (v/v).
Results and discussion
Adsorption coefficientopen allclose all
- Type:
- Koc
- Value:
- 0.8
- pH:
- 2
- Temp.:
- 25 °C
- Key result
- Type:
- log Koc
- Value:
- -0.09
- pH:
- 2
- Temp.:
- 25 °C
- Type:
- Koc
- Value:
- 0.3
- pH:
- 9
- Temp.:
- 25 °C
- Key result
- Type:
- log Koc
- Value:
- -0.54
- pH:
- 9
- Temp.:
- 25 °C
Results: HPLC method
- Details on results (HPLC method):
- - Retention times of reference substances used for calibration: Refer to "Any other information on materials and methods"
- Details of fitted regression line (log k' vs. log Koc): r²: 0.9544 - 0.9558
- Average retention data for test substance: 2.410 min (pH2), 2.380 min (pH9)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Remarks:
- No validity criteria defined in the guideline.
- Conclusions:
- The substance has a low potential for adsorption (log Koc < 3).
- Executive summary:
The adsorption coefficient (Koc) of the test item was determined according to OECD 121 guideline and EEC-guideline 2001/59/EEC C.19. Nine calibration substances were injected into an HPLC-system under the same analytical conditions (column temperature 25 °C) as the test item. A calibration curve was created by using the measured retention times (log k'-values) and the known log Koc-values of the calibration substances for linear regression. At pH 2 and pH 9 the measured log k'-values of the test item were outside at the lower end of the calibrated log k'-range. Therefore, the log Koc values of the test item were extrapolated from the resulted calibration curves.
The resulting log Koc values (-0.09 at pH2 and -0.54 at pH9) indicate a low potential for adsorption.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
