Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
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EC number: 309-984-9 | CAS number: 101896-26-8 A residue from the distillation of crude benzole to remove benzole fronts. Composed primarily of benzene, toluene and xylenes boiling in the range of approximately 75°C to 200°C (167°F to 392°F).
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Does not meet important criteria of today standard methods
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicological Studies of Certain Alkylated Benzenes and Benzene
- Author:
- Wolf MA, Rowe VK, McCollister DD, Hollingsworth RL, and Oyen F
- Year:
- 1 956
- Bibliographic source:
- Arch. Ind. Health 14, 387-398
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Primary eye irritation test; pre-guideline study
- GLP compliance:
- not specified
- Remarks:
- pre-GLP study
Test material
- Reference substance name:
- Benzene
- EC Number:
- 200-753-7
- EC Name:
- Benzene
- Cas Number:
- 71-43-2
- Molecular formula:
- C6H6
- IUPAC Name:
- benzene
- Details on test material:
- - Name of test material (as cited in study report): benzene
- Analytical purity: 99.98%
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- other: albino rabits
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: heterogeneous stock raised at the testing facility
- Diet (e.g. ad libitum): commercial diet supplemented with alfalfa hay
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): Two drops were instilled into the right eyeballs of test animals (no further information). - Duration of treatment / exposure:
- single instillation without washing
- Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- no data
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): no data
SCORING SYSTEM: no scoring system used
TOOL USED TO ASSESS EYE LESIONS: fluorescein staining
Results and discussion
Any other information on results incl. tables
Inflammation and slight swelling of the eyelids and questionable or just perceptible transient superficial necrosis in very small areas of the cornea were registered by the authors. No more data are given.
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- In a pre-guideline eye irritation test, inflammation, slight swelling of the eyelids, and barely perceptible transient superficial necrosis of the cornea (small areas) were observed. Findings were not graded using current scoring systems (Wolf, 1956). Based on irritating effects observed in the treated eyes, benzene is considered irritating to the eye (see also European Union Risk Assessment Report - Benzene, Final Approved Version, 2008).
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