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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1997
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study was carried out according to test guidline and under GLP-conditions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1997
Report date:
1997

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
5'-chloro-3-hydroxy-2',4'-dimethoxy-2-naphthanilide
EC Number:
202-182-9
EC Name:
5'-chloro-3-hydroxy-2',4'-dimethoxy-2-naphthanilide
Cas Number:
92-72-8
Molecular formula:
C19H16ClNO4
IUPAC Name:
N-(5-chloro-2,4-dimethoxyphenyl)-3-hydroxy-2-naphthamide

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Limit test:
yes
Total exposure duration:
96 h

Test conditions

Hardness:
2.1 - 2.5 mmol Ca2+ + Mg2+/L
Test temperature:
21.7 - 22.0°C
pH:
7.7 -7.9
Dissolved oxygen:
7.3 - 8.8 mg/L
Nominal and measured concentrations:
0/10000 mg/L (nominal)
0/0.33 mg/L (mean of measured conc.)
Details on test conditions:
use of reconstituted water, composition acc. to ISO/DIS 7346/1
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.33 mg/L
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: lethality value based on loading rates (nominal conc.); mean value of measured concentrations: 0.33 mg/L
Duration:
96 h
Dose descriptor:
LC100
Remarks on result:
other: not determined

Applicant's summary and conclusion

Conclusions:
67/548/EEC: Acute aquatic toxicity: no classification
1272/2008/EC: Acute aquatic toxicity: no classification
Executive summary:

No lethality was observed up to the solubility limit of Naphtol AS-ITR. The 10000 mg/L (0.33 mg/L*) group exhibited reversible symptoms.

* = value based on the mean of analytically determined values