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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: according to guideline and GLP, no deviations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
2,4-di-tert-butylphenyl diphenyl phosphite; 2-tert-butylphenyl diphenyl phosphite; 4-tert-butylphenyl diphenyl phosphite; triphenyl phosphite
EC Number:
941-802-9
Molecular formula:
Unspecified
IUPAC Name:
2,4-di-tert-butylphenyl diphenyl phosphite; 2-tert-butylphenyl diphenyl phosphite; 4-tert-butylphenyl diphenyl phosphite; triphenyl phosphite
Test material form:
other: yellowish liquid
Details on test material:
expiration date April 30, 2003

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
before test start:
fourfould samples from each test meium (without daphnids), fourfold samples from control (without daphnids)
after 48 hours:
fourfould samples from each test meium, fourfold samples from control

Test solutions

Vehicle:
no
Details on test solutions:
Test water:
Reconstituted water: analytical grade salts were dissolved deionized water to obtain the following nominal concentrations:
CaCl2 X 2H2O 2.0 mmol/L (= 294 mg/L)
MgSO4 X 7H2O 0.5 mmol/L (=123 mg/L)
NaHCO3 0.75 mmol/L (= 65 mg/L)
KCl 0.075 mmol/L (= 5.8 mg/L)
Alkalinity 0.8 mmol/L
Ratio of Ca : Mg 4 : 1 (based on molarity)
Na:K 10 : 1 (based on molarity)


WAF preparation:
5 individual preparaed stocksolutions 5, 6, 50, 160, 500mg in 5L test water)
ultrasonic treatment for 15 minutes and intense stirring for 4 days (dark, room temperature)
saturated aqueous dispersions were filtered through a membrane filter (Schleicher & Schuelll, Type NC20, pore size 0.2 microm) before the strat of the test

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
clone of the species Daphnia magna Straus. A clone of this species was originally supplied by the University of Sheffield/UK in 1992, defined from the supplier as clone 5. Since this date the clone is bred in the laboratories of RCC in reconstituted water of the quality identical to the water quality used in the tests (regarding pH, main ions and total hardness) and under temperature and light conditions identical to those of the tests (see below).
At the start of the test the used test organisms were 6-24 hours old and were not first brood progeny.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.5mmol (250 mg/L) as CaCO3
Test temperature:
21-22°C
pH:
7.8-8
Dissolved oxygen:
>= 8mg/L
Nominal and measured concentrations:
WAF loading rates: 1, 3.2, 10, 32, 100 mg/L
measured: < limit of quantitation (< 16.7 microg/L)
Details on test conditions:
Light conditions:
A 16-hour light to 8-hour darkness photoperiod with a 30 minute transition period (light intensity at light period between 200 and 1200 Lux).
Feeding: None

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
57 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other: WAF
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other: WAF
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Remarks on result:
other: WAF
Details on results:
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions throughout the entire test duration.
EC50 (24h) 57 mg/L
EC0/NOEC (24h) 32 mg/L
EC100(24h) 100mg/L

Applicant's summary and conclusion