1,1-dimethoxytrimethylamine

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation

Data source

Reference

Reference Type:

publication

Title:

The ecotoxicity of ionic liquids and traditional organic solvents on microalga Selenastrum capricornutum

Author:

Cho, C.-W. et al.

Year:

2008

Bibliographic source:

Ecotoxicology and Environmental Safety 71: 166-171

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

IUPAC Name:

methanol

Details on test material:

- Name of test material (as cited in study report): Methanol, CAS-No 67-56-1
- Analytical purity: >99.5 %

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: green alga
- Strain: ATCC-22662
- Source (laboratory, culture collection): National Institute Environmental Research, Korea
- Age of inoculum (at test initiation): 7 days
- Method of cultivation: The flasks then were placed on a shaker incubator at 170 rpm and 25°C, with 24 h light supplied via worm white fluorescent tubes, with an average illumination of 30 ± 5µE/m2s.


ACCLIMATION
- Acclimation period:
- Culturing media and conditions (same as test or not): Stock alga was cultivated in 250 mL Erlenmeyer flasks, containing 200 mL sterilized nitrate-enriched BBM medium prepared in triple distilled water, to avoid nitrogen limitation during the high-density culture. The culture flask was agitated on a shaker at 170 rpm, and bubbled with air (1 vvm), without sparger. Light was continuously supplied, with an average of 30 ±5 µE/m2s, using warm-white fluorescent tubes located on top of the shaker. All the flasks were maintained in the shaker incubator at 25 ± 5°C for 7 days.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

25°C

Nominal and measured concentrations:

no details

Details on test conditions:

TEST SYSTEM

Experiments were performed in 250 mL Erlenmeyer flasks containing 55 mL of sterilized culture medium, inoculated with 5 mL samples of 7 day cultured algae. Solutions of organic solvents were subsequently added to the test flasks. The organic solvents tested were completely miscible with water, forming a homogeneous phase. Concentrations were adjusted in the range between 0.1 M and 1.26 µM to obtain complete concentration-response curves. At each determined exposure date, the optical density of the algal biomass was estimated at 438 nm using a spectrophotometer. The dry cell weight, corresponding to the optical density, was determined from the linear relationship; dry cell weight (g/L) = 0.329 x optical density. All experiments were performed in duplicate, but the control experiments were conducted in triplicate.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

ca. 22 000 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth rate