Methylurea

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 8

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methylurea

EC Number:

209-935-0

EC Name:

Methylurea

Cas Number:

598-50-5

Molecular formula:

C2H6N2O

IUPAC Name:

methylurea

Details on test material:

- Name of test material (as cited in study report): Methylharnstoff technisch

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

17 h

Test conditions

Test temperature:

22 ± 1 °C

pH:

4.8 - 7.3

Nominal and measured concentrations:

Nominal test substance concentrations:
156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Erlenmeyer vessels (300 ml)
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

6 532 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

7 854 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition