Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 8
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methylurea
EC Number:
209-935-0
EC Name:
Methylurea
Cas Number:
598-50-5
Molecular formula:
C2H6N2O
IUPAC Name:
methylurea
Details on test material:
- Name of test material (as cited in study report): Methylharnstoff technisch

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
17 h

Test conditions

Test temperature:
22 ± 1 °C
pH:
4.8 - 7.3
Nominal and measured concentrations:
Nominal test substance concentrations:
156.25, 312.5, 625, 1250, 2500, 5000, 7500 and 10000 mg/l
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer vessels (300 ml)
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

Results and discussion

Effect concentrationsopen allclose all
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
6 532 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
7 854 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition

Applicant's summary and conclusion