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Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
04/06/2010 to 01/07/2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
not specified
GLP compliance:
yes
Type of method:
flask method
Water solubility:
38.9 g/L
Conc. based on:
test mat.
Loading of aqueous phase:
191.6 g/L
Incubation duration:
24 h
Temp.:
20 °C
pH:
7.9
Remarks on result:
other: calculated based on the measured weight% assuming a density of 1 g/mL
Water solubility:
3.89 other: % w/w
Conc. based on:
test mat.
Loading of aqueous phase:
191.6 g/L
Incubation duration:
24 h
Temp.:
20 °C
pH:
7.9
Remarks on result:
other: Calculated from the titration for boric oxide

Preliminary Test:

Weight of test item used = 0.1006

Temperature of test = 20 °C

Total Volume of distilled water added (mL)

0.1

0.5

1

2

10

100

>100

Was test item totally dissolved? (y/n)

No

No

No

No

Yes

N/A

N/A

Approximate solubility (g/L)

> 1000

1000 - 200

200 - 100

100 - 50

50 - 10

10 - 1

< 1

Shake Flask Test: 

Based on the preliminary test the water solubility of the test item is from 10 – 50 g/L. The MSDS for the test item stated that the water solubility was 38 g/L which is within this range. The amount of test item required for 1 L of water would be 5 x 38 = 190 g and as this test is done in triplicate the total amount of test item is 190 x 3 = 570 g. However based on the approximate amount of test item available the maximum weight of test item available for each replicate was19 g. Based on the MSDS solubility of 38 g/L then the amount of water required for 19 g of test item would be: (19/5)/38 x1000 = 100 mL

Approximately 19 g of test item was then accurately weighed into each of three flasks, 100 mL of distilled water was added to each and the flasks were then placed in a temperature controlled shaker at 30°C. Testing was then carried out.

Shake Flask Details:

Replicate

Number

Weight of test item used (g)

Volume of distilled water added (mL)

Number of days shaking

pH of aqueous solution after shaking, 24 hours in 20°C water bath and centrifugation and filtration

Replicate 1

19.1588

100

1

7.86

Replicate 2

19.0759

100

2

7.93

Replicate 3

19.0508

100

3

7.88

After removal from the shaker the test item solutions were placed in a water bath, set at 20°C, for 24 hours prior to centrifugation, filtration and analysis. However due to a delay in the analysis the centrifuged and filtered aqueous test item solutions were placed back in the 20°C water bath and kept at temperature until analysed.

Water Solubility Solution Analysis Results:

Replicate

Number

Volume of aqueous test item solution taken (mL)

Weight of aqueous test item solution taken (g)

Volume of 0.5N hydrochloric acid added (mL)

Volume of 0.5N sodium hydroxide added at first titration end point (mL)

Potassium oxide content (% w/w)

Potassium pentaborate content (% w/w)

Volume of 0.5N sodium hydroxide added at second end point (after addition of mannitol) (mL)

Boric oxide content (% w/w)

Potassium pentaborate content (% w/w)

Replicate 1

5

5.0929

25

23.3

0.66

4.09

6.75

2.31

3.90

Replicate 2

10

10.1624

25

21.90

0.65

4.07

13.50

2.32

3.91

Replicate 3

10

10.1936

25

21.95

0.64

3.99

13.40

2.29

3.86

 

 

 

 

Mean =

0.65

4.05

Mean =

2.31

3.89

 

 

 

 

SD =

0.0087

0.054

SD =

0.013

0.021

 

 

 

 

%RSD =

1.33

1.33

%RSD =

0.55

0.55

Conclusions:
The resulting solutions from the water solubility testing were analysed for active content using British Standard Method BS 5688 Part 8. The mean water solubility was found to be 3.89 % w/w (38.9 g/L) (calculated from the titration for boric oxide).
Executive summary:

The water solubility was analysed following EU Method A.6 under GLP comliance. Flask method was used. The resulting solutions from the water solubility testing were analysed for active content using British Standard Method BS 5688 Part 8. The mean water solubility was found to be 3.89 % w/w (38.9 g/L assuming a density of 1 g/mL) at 20 °C (calculated from the titration for boric oxide).

Description of key information

The water solubility was analysed following EU Method A.6 under GLP comliance. Flask method was used. The resulting solutions from the water solubility testing were analysed for active content using British Standard Method BS 5688 Part 8. The mean water solubility was found to be 3.89 % w/w (38.9 g/L assuming a density of 1 g/mL) at 20 °C (calculated from the titration for boric oxide).

Key value for chemical safety assessment

Water solubility:
38.9 g/L
at the temperature of:
20 °C

Additional information